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Series GSE50821 Query DataSets for GSE50821
Status Public on May 08, 2014
Title Restoring Systemic GDF11 Levels Reverses Age-Related Dysfunction in Mouse Skeletal Muscle
Organism Mus musculus
Experiment type Expression profiling by array
Summary In this study, we investigated signaling pathways in Skeletal muscle precursors that are altered with aging and age-related deficits in muscle regenerative potential. We performed fluorescence activated cell sorting (FACS) to obtain highly purified skeletal muscle satellite cells from young, middle-aged and old mice.
Parabiosis experiments indicate that impaired regeneration in aged mice is reversible by exposure to a young circulation, suggesting that young blood contains humoral "rejuvenating" factors that can restore regenerative function. Here, we demonstrate that the circulating protein growth differentiation factor 11 (GDF11) is a rejuvenating factor for skeletal muscle. Supplementation of systemic GDF11 levels, which normally decline with age, by heterochronic parabiosis or systemic delivery of recombinant protein, reversed functional impairments and restored genomic integrity in aged muscle stem cells (satellite cells). Increased GDF11 levels in aged mice also improved muscle structural and functional features and increased strength and endurance exercise capacity. These data indicate that GDF11 systemically regulates muscle aging and may be therapeutically useful for reversing age-related skeletal muscle and stem cell dysfunction.
Overall design We used Affymetrix Mouse Genome array to identify global transcriptional changes associated with age in skeletal muscle precursors.
Contributor(s) Sinha M, Jang YC, Oh J, Khong D, Wu EY, Manohar R, Miller C, Regalado SG, Loffredo F, Pancoast JR, Hirshman MF, Lebowitz J, Shadrach JL, Cerletti M, Kim MJ, Serwold T, Goodyear LJ, Rosner B, Lee RT, Wagers AJ
Citation(s) 24797481
Submission date Sep 12, 2013
Last update date Feb 11, 2019
Contact name Amy J Wagers
Phone +1-617-309-2590
Organization name Joslin diabetes center and Harvard University
Department Stem Cell and Regenerative Biology
Lab Stem Cell and Regenerative Biology
Street address One Joslin place
City Boston
State/province MA
ZIP/Postal code 02215
Country USA
Platforms (1)
GPL1261 [Mouse430_2] Affymetrix Mouse Genome 430 2.0 Array
Samples (14)
GSM1230351 Young 1
GSM1230352 Young 2
GSM1230353 Young 3
BioProject PRJNA219090

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Supplementary file Size Download File type/resource
GSE50821_RAW.tar 47.4 Mb (http)(custom) TAR (of CEL)
Raw data provided as supplementary file
Processed data included within Sample table

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