|
| Status |
Public on Sep 09, 2014 |
| Title |
Expression data from activated NK cells [RNA] |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
|
| Summary |
Natural Killer (NK) cells present natural cytotoxicity against tumor cells, although their activity is increased after activation. NK cell activation depends on a complex intracellular signaling process mediated by activating and inhibitory receptors and the functional outcome depends on the integration of the activating and inhibitory signals received. Soluble cytokines and/or ligands on target cells bind the NK cell receptors, and hence, influence the final NK cell response: attack versus ignorance.
We used microarrays to detail the global programme of gene expression underlying NK cell activation by IL-2, a MHC-I-deficient target cell (K562)+IL-2 and an EBV-target cell (R69).
|
| |
|
| Overall design |
PBLs from 4 different donors were activated by 100 U/ml IL-2; K562+IL-2 or R69 cells. After 5 days we obtained RNA and miRNA from naïve NK cells or from NK cells activated with the above mentioned stimuli, with more than 90% of purity. The 16 RNA samples were used to generate cDNA libraries that were hybridized on Human Gene 1.1ST arrays (Affymetrix) and analyzed with the Affymetrix Gene Chip Command Console Software v3.0 (AGCC 3.0, Affymetrix®) and the Expression Console Software v1.1 (Affymetrix®).
|
| |
|
| Contributor(s) |
Villalba M, Pardo J |
| Citation(s) |
24440757 |
| Submission date |
Sep 12, 2013 |
| Last update date |
Nov 08, 2016 |
| Contact name |
martin villalba |
| E-mail(s) |
martin.villalba@inserm.fr
|
| Phone |
33467330465
|
| Organization name |
Institut for Research in Biotherapy of Montpellier
|
| Department |
INSERM U1040
|
| Street address |
CHU Montpellier Hôpital Saint-Eloi 80, av. Augustin Fliche.
|
| City |
montpellier |
| ZIP/Postal code |
34295 |
| Country |
France |
| |
|
| Platforms (1) |
| GPL11532 |
[HuGene-1_1-st] Affymetrix Human Gene 1.1 ST Array [transcript (gene) version] |
|
| Samples (16)
|
|
| This SubSeries is part of SuperSeries: |
| GSE50840 |
Expression data from activated NK cells |
|
| Relations |
| BioProject |
PRJNA219135 |
Less...
More...