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Series GSE50895 Query DataSets for GSE50895
Status Public on Jun 25, 2014
Title Gene expression profiling of human IL-10+ B-cells
Organism Homo sapiens
Experiment type Expression profiling by array
Summary Following combined stimulation through Toll-like receptor (TLR)-9 and the B-cell receptor (BCR), human B cells were sorted based on IL-10 expression.
Microarray analysis showed that just ~0.7% of genes were differentially expressed between IL-10- and IL-10+ B-cells. However, connectivity map analysis revelaed that the IL-10+ cells were those undergoing differentiation to germincal centre B cells, and we identified a CD11c- B-cell subset that was enriched in cells capable of producing IL-10
 
Overall design B-cells were isolated using the Dynabeads Untouched Human B-cells kit, stimulated during 48 hours and then sorted based on IL-10 using secretion assay kit and cell sorter.
 
Contributor(s) Lin W, Duan K, Lum J, Zolezzi F, Poidinger M, Calbo S
Citation(s) 25080484
Submission date Sep 16, 2013
Last update date Feb 18, 2019
Contact name Michael Poidinger
E-mail(s) michael_poidinger@immunol.a-star.edu.sg
Phone +6564070528
URL http://www.sign.a-star.edu.sg
Organization name Singapore Immunology Network
Department Bioinformatics
Street address #04-06, 8A Biomedical Grove
City Singapore
ZIP/Postal code 138648
Country Singapore
 
Platforms (1)
GPL5175 [HuEx-1_0-st] Affymetrix Human Exon 1.0 ST Array [transcript (gene) version]
Samples (10)
GSM1232094 CD19-IL10neg1
GSM1232095 CD19-IL10pos1
GSM1232096 CD19-IL10neg2
Relations
BioProject PRJNA219309

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE50895_RAW.tar 251.4 Mb (http)(custom) TAR (of CEL)
Processed data included within Sample table

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