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Status |
Public on Jun 25, 2014 |
Title |
Gene expression profiling of human IL-10+ B-cells |
Organism |
Homo sapiens |
Experiment type |
Expression profiling by array
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Summary |
Following combined stimulation through Toll-like receptor (TLR)-9 and the B-cell receptor (BCR), human B cells were sorted based on IL-10 expression. Microarray analysis showed that just ~0.7% of genes were differentially expressed between IL-10- and IL-10+ B-cells. However, connectivity map analysis revelaed that the IL-10+ cells were those undergoing differentiation to germincal centre B cells, and we identified a CD11c- B-cell subset that was enriched in cells capable of producing IL-10
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Overall design |
B-cells were isolated using the Dynabeads Untouched Human B-cells kit, stimulated during 48 hours and then sorted based on IL-10 using secretion assay kit and cell sorter.
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Contributor(s) |
Lin W, Duan K, Lum J, Zolezzi F, Poidinger M, Calbo S |
Citation(s) |
25080484 |
Submission date |
Sep 16, 2013 |
Last update date |
Feb 18, 2019 |
Contact name |
Michael Poidinger |
E-mail(s) |
michael_poidinger@immunol.a-star.edu.sg
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Phone |
+6564070528
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URL |
http://www.sign.a-star.edu.sg
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Organization name |
Singapore Immunology Network
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Department |
Bioinformatics
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Street address |
#04-06, 8A Biomedical Grove
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City |
Singapore |
ZIP/Postal code |
138648 |
Country |
Singapore |
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Platforms (1) |
GPL5175 |
[HuEx-1_0-st] Affymetrix Human Exon 1.0 ST Array [transcript (gene) version] |
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Samples (10)
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Relations |
BioProject |
PRJNA219309 |