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Series GSE51040 Query DataSets for GSE51040
Status Public on Nov 01, 2016
Title Host miRNA molecular signatures in the brains of mice are associated with acute herpes simplex virus 1 encephalitis
Organism Mus musculus
Experiment type Expression profiling by array
Summary Transcriptional profiling of mouse brain regions (microdissection of cerebellum and hippocampus regions) comparing control, untreated brain tissue with HSV-1 infected brain tissue. Two viral tires, 10^3 and 10^6 plaque forming units of HSV-1 strain F, were intracerebrally inoculated into the brain of SJL/J strain of mice. The goal was to assess global transcriptional dynamics in HSV-1 infected mice, a model of herpes simplex encephalitis, as compared to controls (either PBS or Vero cell suspension). The microarray data identified fluctuations in gene expression where many of these genes were also potential targets of miRNA-200 and miR-183 family members which were themselves upregulated within the same brain samples.
 
Overall design Eight experimental conditions were compared: 1) hippocampus 10^3 plaque forming units of HSV-1 infected brain samples were compared to PBS control; 2) hippocampus 10^3 plaque forming units of HSV-1 infected brain samples were compared to Vero controls; 3) hippocampus 10^6 plaque forming units of HSV-1 infected brain samples were compared to PBS; 4) hippocampus 10^6 plaque forming units of HSV-1 infected brain samples were compared to Vero controls; 5) cerebellum 10^3 plaque forming units of HSV-1 infected brain samples were compared to PBS control; 6) cerebellum 10^3 plaque forming units of HSV-1 infected brain samples were compared to Vero controls; 7) cerebellum 10^6 plaque forming units of HSV-1 infected brain samples were compared to PBS; 8) cerebellum 10^6 plaque forming units of HSV-1 infected brain samples were compared to Vero controls. Biological replicates: 2 cerebellum 10^3 HSV-1 infected brain samples; 2 hippocampus 103 HSV-1 infected brain samples; 2 cerebellum 10^6 HSV-1 infected brain samples; 2 hippocampus 106 HSV-1 infected brain samples; 1 Vero control sample, 1 PBS control sample. Dye swaps were performed for all array samples except for sample 251486812045_6LHippocmapus_10^3_555.txt.
 
Contributor(s) Majer A, Caligiuri KA, Gale KK, Niu Y, Phillipson CS, Booth TF, Booth SA
Citation(s) 28045967
Submission date Sep 20, 2013
Last update date May 10, 2018
Contact name Stephanie A Booth
E-mail(s) Stephanie.Booth@phac-aspc.gc.ca
Organization name Public Health Agency of Canada
Department Molecular PathoBiology
Street address 1015 Arlington Street
City Winnipeg
State/province Manitoba
ZIP/Postal code R3E 3R2
Country Canada
 
Platforms (1)
GPL4134 Agilent-014868 Whole Mouse Genome Microarray 4x44K G4122F (Feature Number version)
Samples (23)
GSM1236437 Cerebellum_HSV-1_10^3_555_vs_Control_647_rep 1_1
GSM1236438 Cerebellum_HSV-1_10^3_647_vs_Control_555_rep 1_1
GSM1236439 Cerebellum_HSV-1_10^3_555_vs_Control_647_rep 1_2
Relations
BioProject PRJNA219738

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE51040_RAW.tar 311.8 Mb (http)(custom) TAR (of TXT)
Processed data included within Sample table
Processed data provided as supplementary file
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