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| Status |
Public on May 14, 2014 |
| Title |
Proteomic and Genomic Analysis of transcription factor TFII-I reveals insight into the response to cellular stress |
| Organism |
Homo sapiens |
| Experiment type |
Genome binding/occupancy profiling by high throughput sequencing
|
| Summary |
We expressed biotin-tagged transcription factor TFII-I (GTF2I) in K562 cells and subjected crosslinked chromatin to streptavidin mediated pull down. We subjected the isolated DNA to Illumina sequencing. We identified 18920 TFII-I binding peaks. Nearest gene analysis revealed 5886 genes associated with a TFII-I binding peak in proliferating K562 cells.
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| Overall design |
We generated two K562 single cell clones stably expressing bio-tagged TFII-I, and K562 cells expressing only the BirA protein biotin ligase from E.coli. These three clones were subjected to streptavidin mediated pull down and Illumina sequencing.
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| Contributor(s) |
Bungert J, Strouboulis J |
| Citation(s) |
24875474 |
| Submission date |
Sep 20, 2013 |
| Last update date |
May 15, 2019 |
| Contact name |
Giorgio L Papadopoulos |
| Organization name |
BSRC 'Alexander Fleming'
|
| Department |
Molecular Oncology
|
| Lab |
John Strouboulis Lab
|
| Street address |
34 Fleming Street
|
| City |
Athens |
| State/province |
Attiki |
| ZIP/Postal code |
16672 |
| Country |
Greece |
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| Platforms (1) |
| GPL10999 |
Illumina Genome Analyzer IIx (Homo sapiens) |
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| Samples (3) |
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| Relations |
| BioProject |
PRJNA220979 |
| SRA |
SRP030131 |
