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Series GSE5112 Query DataSets for GSE5112
Status Public on Aug 01, 2006
Title Gene expression induced by flagellin stimulation in IECs
Organism Mus musculus
Experiment type Expression profiling by array
Summary Microarray analysis of IECs from Tlr5+/+ and Tlr5–/– mice stimulated with either medium alone or flagellin (1 µg/ml); to elucidate TLR5-mediated immune responses in IECs.
Keywords: ordered
Overall design IECs from Tlr5+/+ and Tlr5–/– mice were treated with or without flagellin (1 ug/ml) for 4 h. Total RNA was extracted with an RNeasy kit (Qiagen), and purified using an Oligotex mRNA Kit (Pharmacia). Fragmented and biotin-labeled cDNA was synthesized from 100 ng purified mRNA using the Ovation Biotin System (Nugen), according to the manufacturer’s protocol. cDNA was hybridized to Affymetrix Murine Genome 430 2.0 microarray chips (Affymetrix) according to the manufacturer’s instructions. Hybridized chips were stained, washed and scanned using a GeneArray Scanner (Affymetrix). Data analysis was performed with Microarray Suite software (Version 5.0, Affymetrix) and GeneSpring software (Silicon Genetics).
Contributor(s) Uematsu S
Citation(s) 16829963
Submission date Jun 21, 2006
Last update date Feb 11, 2019
Contact name Satoshi Uematsu
Phone +81-6-6879-8303
Fax +81-6-6879-8305
Organization name Research Institue for Micrbial Diseases Osaka University
Department Host Defense
Street address 3-1, Yamada-oka
City Suita
State/province Osaka
ZIP/Postal code 565-0871
Country Japan
Platforms (1)
GPL1261 [Mouse430_2] Affymetrix Mouse Genome 430 2.0 Array
Samples (4)
GSM115320 Wild IECs 4h
GSM115321 Wild_IEC_unstim
GSM115322 TLR5KO IEC unstim
This SubSeries is part of SuperSeries:
GSE5119 Gene expression induced by flagellin stimulation in IECs and CD11c+ LPCs
BioProject PRJNA104511

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