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Series GSE51190 Query DataSets for GSE51190
Status Public on Jun 16, 2014
Title Transcriptional network analysis in muscle reveals AP-1 as a partner of PGC-1α in the regulation of the hypoxic gene program [microarray: kD_AP1]
Organism Mus musculus
Experiment type Expression profiling by array
Summary Skeletal muscle tissue shows an extraordinary cellular plasticity, but the underlying molecular mechanisms are still poorly understood. Here we use a combination of experimental and computational approaches to unravel the complex transcriptional network of muscle cell plasticity centered on the peroxisome proliferator-activated receptor γ coactivator 1α (PGC-1α), a regulatory nexus in endurance training adaptation. By integrating data on genome-wide binding of PGC-1α and gene expression upon PGC-1α over-expression with comprehensive computational prediction of transcription factor binding sites (TFBSs), we uncover a hitherto underestimated number of transcription factor partners involved in mediating PGC-1α action. In particular, principal component analysis of TFBSs at PGC-1α binding regions predicts that, besides the well-known role of the estrogen-related receptor α (ERRα), the activator protein-1 complex (AP-1) plays a major role in regulating the PGC-1α-controlled gene program of hypoxia response. Our findings thus reveal the complex transcriptional network of muscle cell plasticity controlled by PGC-1α.
 
Overall design We used microarrays to detect changes in gene expression in C2C12 cells following PGC-1alpha over-expression and either Atf3, Fos, Jun or control knockdown. We used 2 biological replicates for each condition.
 
Contributor(s) Baresic M, Salatino S, van Nimwegen E, Handschin C, Kupr B
Citation(s) 24912679
Submission date Sep 26, 2013
Last update date Jun 17, 2014
Contact name Christoph Handschin
Organization name Biozentrum, University of Basel
Street address Klingelbergstrasse 50/70
City Basel
ZIP/Postal code 4056
Country Switzerland
 
Platforms (1)
GPL10740 [MoGene-1_0-st] Affymetrix Mouse Gene 1.0 ST Array [probe set (exon) version]
Samples (10)
GSM1240362 Atf3 KD, PGC1a OV, biol. rep. 1
GSM1240363 Ctrl KD, GFP OV, biol. rep. 1
GSM1240364 Ctrl KD, PGC1a OV, biol. rep. 1
This SubSeries is part of SuperSeries:
GSE51191 Transcriptional network analysis in muscle reveals AP-1 as a partner of PGC-1α in the regulation of the hypoxic gene program
Relations
BioProject PRJNA221570

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE51190_RAW.tar 42.0 Mb (http)(custom) TAR (of CEL)
GSE51190_log2_expr_kD_AP1_promoters.txt.gz 2.0 Mb (ftp)(http) TXT
Raw data provided as supplementary file
Processed data are available on Series record

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