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| Status |
Public on Sep 26, 2014 |
| Title |
Gene expression analysis in the lungs of Fra-2 Transgenic mice |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
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| Summary |
The aim of this experiment is to identify novel gene candidates being regulated in Fra-2 overexpressing mice. Fra-2 over-expressing mice (Fra-2 TG) develop vascular remodeling and pulmonary fibrosis. Fra-2, as a trascription factor, is responsible for downstream target genes expression. Comparison of the mRNA expressed from WT and Fra-2 TG animals will help to identify Fra-2 target genes. The microarray analysis will be performed in mRNA isolated from lung homogenate. The lungs of the mice were extensively perfused. The right part was snap-froen and transfered to (-80°C). The total RNA was isolated and quantified.
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| Overall design |
Comparison of transgenes vs. wild-types. Hybridization of samples from 4 wild-type and 4 transgene animals = 8 single-color hybridizations
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| Contributor(s) |
Jochen W, Valentina B |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| Submission date |
Sep 26, 2013 |
| Last update date |
Jul 19, 2017 |
| Contact name |
Jochen Wilhelm |
| Organization name |
Justus-Liebig University Giessen
|
| Department |
Institute for Lung Health
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| Lab |
ILH Genomics
|
| Street address |
Aulweg 132
|
| City |
Giessen |
| State/province |
Hessen |
| ZIP/Postal code |
35392 |
| Country |
Germany |
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| Platforms (1) |
| GPL13912 |
Agilent-028005 SurePrint G3 Mouse GE 8x60K Microarray (Feature Number version) |
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| Samples (8)
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| Relations |
| BioProject |
PRJNA221761 |
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