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| Status |
Public on Aug 28, 2006 |
| Title |
Hoxb1 mutant mRNA levels in rhombomere 4 at E10.5 |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
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| Summary |
Hoxb1 is required for proper specification of rhombomere 4 and the facial motor neurons. This study analyzed gene expression in the corresponding hindbrain segment of E10.5 mutant embryos. Several genetic pathways were found altered, including transcription factors such as Phox2b, Gata3, Nkx2-2 and Nkx6-1.
Keywords: hindbrain development, rhombomere 4, Hoxb1
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| Overall design |
Each of the three mutant and control samples was an independent biological replicate. Pools of r4 segments from multiple embryos were snap frozen on dry ice and stored at -80ºC. Total RNA was isolated, processed with standard Affymetrix protocols and hybridized to GeneChip Mouse Expression Sets 430A.
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| Contributor(s) |
Tvrdik P, Arenkiel BR, Gaufo GO, Weiss RB, Capecchi MR |
| Citation(s) |
16890163 |
| Submission date |
Jun 22, 2006 |
| Last update date |
Jan 08, 2019 |
| Contact name |
Petr Tvrdik |
| E-mail(s) |
petr.tvrdik@genetics.utah.edu
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| Phone |
(801) 581-7097
|
| Organization name |
University of Utah
|
| Department |
Human Genetics
|
| Lab |
Capecchi Lab
|
| Street address |
15 N 2030 E
|
| City |
Salt Lake City |
| State/province |
UT |
| ZIP/Postal code |
84112 |
| Country |
USA |
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| Platforms (1) |
| GPL339 |
[MOE430A] Affymetrix Mouse Expression 430A Array |
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| Samples (6)
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| Relations |
| BioProject |
PRJNA96627 |
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