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Series GSE5128 Query DataSets for GSE5128
Status Public on Sep 01, 2006
Title Gene Expression Biomarkers for Predicting Liver Tumors in Two-Year Rodent Bioassays
Organism Mus musculus
Experiment type Expression profiling by array
Summary Two-year rodent bioassays play a central role in evaluating both the carcinogenic potential of a chemical and generating quantitative information on the dose-response behavior for chemical risk assessments. The bioassays involved are expensive and time-consuming, requiring nearly lifetime exposures (two years) in mice and rats and costing $2 to $4 million per chemical. Since there are approximately 80,000 chemicals registered for commercial use in the United States and 2,000 more are added each year, applying animal bioassays to all chemicals of concern is clearly impossible. To efficiently and economically identify carcinogens prior to widespread use and human exposure, alternatives to the two-year rodent bioassay must be developed. In this study, animals were exposed for 13 weeks to two chemicals that were positive for liver tumors in the two-year rodent bioassay, two chemicals that were negative for liver tumors, and two vehicle controls. Gene expression analysis was performed on the livers of the animals to assess the potential for identifying gene expression biomarkers that can predict tumor formation in a two-year bioassay following a 13 week exposure.
Keywords: toxicology, chemical carcinogenesis, liver
 
Overall design Five week old female B6C3F1 mice were exposed for 13 weeks to the following treatments: 1) 1,5-Naphthalenediamine, CAS No. 2243-62-1, feed, 2000 ppm, positive liver carcinogen; 2) 2,3-Benzofuran, CAS No. 271-89-6, gavage, 240 mg/kg, positive liver carcinogen; 3) N-(1-naphthyl)ethylenediamine dihydrochloride, CAS No. 1465-25-4, feed, 2000 ppm, negative liver carcinogen; 4) Pentachloronitrobenzene, CAS No. 82-68-8, feed, 8187 ppm, negative liver carcinogen; 5) Feed control; 6) Corn oil gavage control. Feed animals were exposed 7 days/week and gavage animals were exposed 5 days/week (5 ml/kg). Microarray analysis was performed on the livers of three mice per treatment group.
 
Contributor(s) Thomas RS, Pluta LJ, Page TJ, Yang L
Citation(s) 17114358
Submission date Jun 22, 2006
Last update date Feb 11, 2019
Contact name Russell Scott Thomas
E-mail(s) rthomas@thehamner.org
Phone 919-558-1311
Fax 919-558-1300
Organization name The Hamner Institutes for Health Sciences
Street address 6 Davis Drive
City Research Triangle Park
State/province NC
ZIP/Postal code 27709
Country USA
 
Platforms (1)
GPL1261 [Mouse430_2] Affymetrix Mouse Genome 430 2.0 Array
Samples (18)
GSM115680 LiverTissue_GavageTreatment_2,3-Benzofuran_Animal10
GSM115681 LiverTissue_FeedTreatment_N-(1-naphthyl)ethylenediamineDihydrochloride_Animal12
GSM115682 LiverTissue_FeedTreatment_N-(1-naphthyl)ethylenediamineDihydrochloride_Animal14
Relations
BioProject PRJNA96631

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Supplementary file Size Download File type/resource
GSE5128_RAW.tar 104.6 Mb (http)(custom) TAR (of CEL)
Raw data provided as supplementary file

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