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Series GSE51307 Query DataSets for GSE51307
Status Public on Feb 13, 2014
Title Transcriptome-based network analysis reveals a spectrum model of human macrophage activation [miRNA-seq]
Organism Homo sapiens
Experiment type Non-coding RNA profiling by high throughput sequencing
Summary Macrophage activation is associated with profound transcriptional reprogramming. Although much progress has been made in the understanding of macrophage activation, polarization and function, the transcriptional programs regulating these processes remain poorly characterized. We stimulated human macrophages with diverse activation signals, acquiring a dataset of 299 macrophage transcriptomes. Analysis of this dataset revealed a spectrum of macrophage activation states extending the current M1 versus M2-polarization model. Network analyses identified central transcriptional regulators associated with all macrophage activation complemented by regulators related to stimulus-specific programs. Applying these transcriptional programs to human alveolar macrophages from smokers and patients with chronic obstructive pulmonary disease (COPD) revealed an unexpected loss of inflammatory signatures in COPD patients. Finally, by integrating murine data from the ImmGen project we propose a refined, activation-independent core signature for human and murine macrophages. This resource serves as a framework for future research into regulation of macrophage activation in health and disease.
Overall design Since transcriptional programs are further modulated on several levels including miRNAs we assessed the global spectrum of miRNA expression by miRNA-Seq in macrophages stimulated with IFNγ, IL4 or with the combination of TNFα, PGE2 and P3C
Contributor(s) Xue J, Schmidt SV, Sander J, Draffehn A, Krebs W, Quester I, De Nardo D, Gohel TD, Emde M, Schmidleithner L, Ganesan H, Nino-Castro A, Mallmann MR, Labzin L, Theis H, Kraut M, Beyer M, Latz E, Freeman TC, Ulas T, Schultze JL
Citation(s) 24530056
Submission date Sep 30, 2013
Last update date May 15, 2019
Contact name Joachim Schultze
Organization name LIMES (Life and Medical Sciences Center Genomics and Immunoregulation)
Department Genomics and Immunoregulation
Street address Carl-Troll-Strasse 31
City Bonn
State/province NRW
ZIP/Postal code 53115
Country Germany
Platforms (1)
GPL15456 Illumina HiScanSQ (Homo sapiens)
Samples (20)
GSM1242494 M0_0h_rep1_miRNAseq
GSM1242495 M0_0h_rep2_miRNAseq
GSM1242496 M0_0h_rep3_miRNAseq
This SubSeries is part of SuperSeries:
GSE47189 Transcriptome-based network analysis reveals a spectrum model of human macrophage activation
BioProject PRJNA222999
SRA SRP031496

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Supplementary file Size Download File type/resource
GSE51307_GEO_Schultze_miRNA_Seq_processedData_NORM_TRANSF_BR.txt.gz 104.2 Kb (ftp)(http) TXT
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