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| Status |
Public on Jul 07, 2006 |
| Title |
Adjudin-induced germ cell loss from adult rat testes |
| Organism |
Rattus norvegicus |
| Experiment type |
Expression profiling by array
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| Summary |
This is a study to explore the transcriptional changes after Adjudin treatment in adult rat testes at three time points (control, 8 hour and 4 day). Adjudin, [1-(2,4-dichlorobenzyl)-1H-indazole-3-carbohydrazide], is a potential male contraceptive that targets the Sertoli-germ cell interface and causes germ cell depletion from the seminiferous epithelium. Adjudin has been proved to be a useful model to study the mechanisms that regulate junction restructuring in the testis.
Keywords: Sertoli-germ cell adhesion regulation using an Adjudin model
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| Overall design |
Sprague-Dawley rats treated with a single dose of Adjudin at 50 mg/kg b.w. by gavage and terminated after 8 hours (n=2) and 4 days (n=3). Testes from Adjudin-treated rats and untreated (control, n=3) rats were harvested and total RNA were prepared. Standard Affymetrix genechip procedures were followed for the subsequent experiments. Data were analysed in MAS 5.0 and GeneSpring 7.2.
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| Contributor(s) |
Xia W, Mruk DD, Lee WM, Cheng Y |
| Citation(s) |
17332525 |
| Submission date |
Jun 22, 2006 |
| Last update date |
Jul 31, 2017 |
| Contact name |
Weiliang Xia |
| E-mail(s) |
wxia@mail.rockefeller.edu
|
| Phone |
212-327-8738
|
| Fax |
212-327-8733
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| Organization name |
Population Council
|
| Street address |
1230 York Avenue
|
| City |
New York |
| State/province |
NY |
| ZIP/Postal code |
10021 |
| Country |
USA |
| |
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| Platforms (1) |
| GPL1355 |
[Rat230_2] Affymetrix Rat Genome 230 2.0 Array |
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| Samples (8)
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| GSM115952 |
Testis from adult rat treated with 50 mg/kg b.w. Adjudin 8 h-1 |
| GSM115953 |
Testis from adult rat treated with 50 mg/kg b.w. Adjudin 8 h-2 |
| GSM115954 |
Testis from adult rat treated with 50 mg/kg b.w. Adjudin 4 d-1 |
| GSM115955 |
Testis from adult rat treated with 50 mg/kg b.w. Adjudin 4 d-2 |
| GSM115956 |
Testis from adult rat treated with 50 mg/kg b.w. Adjudin 4 d-3 |
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| Relations |
| BioProject |
PRJNA96637 |
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