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Series GSE51427 Query DataSets for GSE51427
Status Public on Jan 01, 2014
Title Eye-Specific Gene Expression following Embryonic Ethanol Exposure in Zebrafish: Roles for Heat Shock Factor 1
Organism Danio rerio
Experiment type Expression profiling by array
Summary Ethanol is a well-known teratogen. While this teratogenic potential is well-characterized clinically, the mechanisms through which ethanol exposure results in developmental defects remain unclear. Here we use the zebrafish model to elucidate eye-specific mechanisms that may underlie ethanol-mediated microphthalmia (reduced eye size), using time-series microarray analysis of gene expression of eye tissues of embryos exposed to 1.5% ethanol vs. untreated embryos. We identified 62 genes differentially expressed in ethanol-treated as compared to control zebrafish eyes from all sampling times over the period of retinal neurogenesis (24-48 hours post-fertilization). Application of the EDGE (extraction of differential gene expression) algorithm identified over 3000 genes differentially expressed over developmental time in ethanol-treated embryo eyes as compared to untreated embryo eyes. These lists included several genes indicating a mis-regulated cellular stress response (heat shock response) due to ethanol exposure. Combined treatment with sub-threshold levels of ethanol and a morpholino (MO) targeting heat shock factor 1 (hsf-1) mRNA resulted in a microphthalmic phenotype, suggesting convergent molecular pathways. Manipulation of the heat shock response by thermal preconditioning partially prevented ethanol-mediated microphthalmia while maintaining Hsf-1 expression. Together these data are consistent with roles for reduced Hsf-1 in mediating microphthalmic effects of embryonic ethanol exposure in zebrafish.
 
Overall design time series, 9 samples, no replicates
 
Contributor(s) Stenkamp D, Kashyap B
Citation(s) 24355176
Submission date Oct 18, 2013
Last update date Jan 25, 2018
Contact name Deborah Lynn Stenkamp
E-mail(s) dstenkam@uidaho.edu
Phone 208-885-8963
Organization name University of Idaho
Department Biological Sciences
Street address 875 Perimeter Drive, MS 3051
City Moscow
State/province Idaho
ZIP/Postal code 83844-3051
Country USA
 
Platforms (1)
GPL1319 [Zebrafish] Affymetrix Zebrafish Genome Array
Samples (9)
GSM1245244 24 hpf pretreatment
GSM1245245 27 hpf control
GSM1245246 27 hpf ethanol
Relations
BioProject PRJNA223154

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE51427_RAW.tar 20.7 Mb (http)(custom) TAR (of CEL, CHP)
Raw data provided as supplementary file
Processed data included within Sample table
Processed data provided as supplementary file

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