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Series GSE51446 Query DataSets for GSE51446
Status Public on Jan 03, 2014
Title Similarities and differences between human activated macrophages II
Organism Homo sapiens
Experiment type Expression profiling by array
Summary We compare, in human macrophages, stimuli considered to induce M1 or M2 type of activation, also termed classical and alternative activation.
This dataset highlights key differences between the M1 and M2 stimuli pointing to the need of a revision of the paradigm.
Macrophages were left untreated or stimulated with IL-4 and IL-13 (prototypic M1 stimuli), Dexamethasone (Glucocorticoid receptor agonist) which has been hitherto considered M2, IL-10 which also falls under the M2 umbrella, and LPS and Interferon Gamma considered M1 stimuli.

Overall design Total RNA obtained from autologous serum monocyte-derived macrophages exposed to:

SAMPLE 1 Untreated
SAMPLE 2 Dexamethasone 100nM
SAMPLE 3 LPS 10 ng/ml
SAMPLE 4 IL-10 20 ng/ml
SAMPLE 5 IL-13 20 ng/ml
SAMPLE 6 IL-4 20 ng/ml
SAMPLE 7 IFNy 20 ng/ml
Contributor(s) Martinez FO, Gordon S
Citation(s) 23293084
Submission date Oct 20, 2013
Last update date Aug 13, 2018
Contact name Fernando O Martinez Estrada
Organization name University of Surrey
Department Faculty of Health & Medical Sciences
Lab Innate Immunity
Street address Stag Hill
City Guildford
State/province Surrey
ZIP/Postal code GU2 7XH
Country United Kingdom
Platforms (1)
GPL10558 Illumina HumanHT-12 V4.0 expression beadchip
Samples (7)
GSM1245954 Untreated
GSM1245955 Dexamethasone 100nM
GSM1245956 LPS 10 ng/ml
This SubSeries is part of SuperSeries:
GSE35495 Genome-wide analysis of human and mouse macrophages, resting and activated
BioProject PRJNA232932

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE51446_RAW.tar 26.2 Mb (http)(custom) TAR
GSE51446_non-normalized.txt.gz 2.3 Mb (ftp)(http) TXT
Raw data are available on Series record
Processed data included within Sample table

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