 |
 |
GEO help: Mouse over screen elements for information. |
|
| Status |
Public on Oct 24, 2013 |
| Title |
Direct Conversion of Human Fibroblasts into Neuronal Restricted Progenitors |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
|
| Summary |
Neuronal restricted progenitors (NRPs) represent a type of transitional intermediate cells that lie between multipotent neural progenitors (NPs) and terminal differentiated neurons during neurogenesis. These NRPs have the ability to self-renew and differentiate into neurons, but not into glial cells, which is considered as an advantage for cellular therapy of human neurodegenerative diseases. However, difficulty in the extraction of highly purified NPRs from normal nervous tissue prevents further studies and applications. In this study, we reported conversion of human fetal dermal fibroblasts into human induced neuronal restricted progenitors (hiNRPs) in seven days by using just three defined factors: Sox2, c-Myc, and either Brn2 or Brn4. The hiNRPs exhibited distinct neuronal characteristics, including cell morphology, multiple neuronal markers expression, self-renewal capacity, and genome-wide transcriptional profile. Moreover, hiNRPs were able to differentiate into various terminal neurons with functional membrane properties, but not glial cells. Direct generation of hiNRPs from somatic cells will provide a new source of cells for cellular replacement therapy of human neurodegenerative diseases.
|
| |
|
| Overall design |
This is a general expression microarray design (NimbleGen platform). It includes 5 samples.
|
| |
|
| Contributor(s) |
Zou Q, Yan Q, Lai L |
| Citation(s) |
24385434 |
| Submission date |
Oct 23, 2013 |
| Last update date |
May 28, 2014 |
| Contact name |
Qingjian Zou |
| E-mail(s) |
zou_qingjian@gibh.ac.cn
|
| Organization name |
Guangzhou Institutes of Biomedicine and Health, CAS
|
| Street address |
190 Kaiyuan Street, Guangzhou Science district
|
| City |
Guangzhou |
| State/province |
Guangdong |
| ZIP/Postal code |
510530 |
| Country |
China |
| |
|
| Platforms (1) |
| GPL16025 |
NimbleGen Homo sapiens Expression Array [100718_HG18_opt_expr] |
|
| Samples (5)
|
|
| Relations |
| BioProject |
PRJNA223534 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE51580_RAW.tar |
24.9 Mb |
(http)(custom) |
TAR (of PAIR) |
| Processed data included within Sample table |
|
|
|
|
 |
Less...
More...