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Series GSE51605 Query DataSets for GSE51605
Status Public on Mar 29, 2014
Title Simple Derivation of Transgene-Free iPS Cells by a Dual Recombinase Approach
Organism Mus musculus
Experiment type Expression profiling by array
Summary Induced pluripotent stem cells (iPSCs) can be derived from somatic cells by the introduction of the transcription factors Oct4, Sox2, Klf4 and cMyc using various methods. Here, we describe a new approach for the derivation of murine iPSCs using a polycistronic non-viral inducible vector integrated into pseudo attP sites via the C31 integrase-mediated site-specific recombination and subsequent vector excision by Cre recombinase.
The pluripotency of the derived iPSCs was proved by in vitro and in vivo tests. The derived transgene-free iPSCs reactivated the endogenous pluripotency genes like e.g. Oct4, Sox2 and Nanog and the global gene expression profiles of iPSCs lines are highly similar to ESCs and distinct from parental murine fibroblasts. We demonstrated the differentiation potential of iPSCs by generation cells of the three germ layers as well as we successfully created germline chimeric mice from transgene-free iPSCs. In this study, we presented an efficient method for the generation of transgene-free iPSCs using dual-recombinase technology.
 
Overall design expression data of iPSCs/ESCs/MEFs
 
Contributor(s) Pertek A, Irmler M, Beckers J, Kühn R
Citation(s) 24677035
Submission date Oct 23, 2013
Last update date Jun 14, 2018
Contact name Johannes Beckers
E-mail(s) beckers@helmholtz-muenchen.de
Organization name Helmholtz Zentrum Muenchen
Department Institute of Experimental Genetics
Street address Ingolstaedter Landstr. 1
City Neuherberg
ZIP/Postal code 85764
Country Germany
 
Platforms (1)
GPL6885 Illumina MouseRef-8 v2.0 expression beadchip
Samples (15)
GSM1249095 iPSC, clone 48, biological replicate 3
GSM1249096 iPSC, clone 48, biological replicate 2
GSM1249097 iPSC, clone 48, biological replicate 1
Relations
BioProject PRJNA224092

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE51605_RAW.tar 3.1 Mb (http)(custom) TAR
GSE51605_non-normalized.txt.gz 1.3 Mb (ftp)(http) TXT
Raw data are available on Series record
Processed data included within Sample table

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