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Series GSE51643 Query DataSets for GSE51643
Status Public on Sep 02, 2014
Title Critical requirement of ERK/MAPK signaling in respiratory tract development
Organism Mus musculus
Experiment type Expression profiling by array
Summary The mammalian genome contains two ERK/MAP kinase kinase genes, Mek1 and Mek2, which encode dual-specificity kinases responsible for ERK/MAP kinase activation. To define the function of ERK/MAPK signaling pathway in lung development, we performed tissue-specific deletions of Mek1 function in a Mek2 null background. Inactivation of both Mek genes in mesenchyme resulted in several phenotypes including giant omphalocele, skeletal defects, pulmonary hypoplasia, abnormal trachea patterning, and death at birth. Microarray analysis with RNA extracted from lungs of E15.5 Dermo1+/Cre, Mek1+/flox;Mek2-/-;Dermo1+/Cre and Mek1flox/flox;Mek2-/-;Dermo1+/Cre embryos was performed to evaluate the molecular impact of the loss of all Mek alleles in mesenchyme on lung development. .
 
Overall design Total RNA was isolated from lungs of E15.5 Dermo1+/Cre embryos (control), from E15.5 Mek1+/flox;Mek2- /-;Dermo1+/Cre embryos (experimental) and from E15.5 Mek1flox/flox;Mek2-/-;Dermo1+/Cre embryos (experimental). Four specimens were analyzed per genotype.
 
Contributor(s) Jeannotte L, Charron J, Boucherat O
Citation(s) 25100655
Submission date Oct 24, 2013
Last update date Jun 14, 2018
Contact name Eric R Paquet
Organization name EPFL
Department Life sciences
Lab Naef lab
Street address Station 15 CH - 1015
City Lausanne
State/province Vaud
ZIP/Postal code 1015
Country Switzerland
 
Platforms (1)
GPL6885 Illumina MouseRef-8 v2.0 expression beadchip
Samples (12)
GSM1250023 E15.5_Lung_Dermo1+/Cre_rep1.
GSM1250024 E15.5_Lung_Dermo1+/Cre_rep2.
GSM1250025 E15.5_Lung_Dermo1+/Cre_rep3.
Relations
BioProject PRJNA224160

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE51643_RAW.tar 3.1 Mb (http)(custom) TAR
GSE51643_non-normalized.txt.gz 1.9 Mb (ftp)(http) TXT
Processed data included within Sample table
Raw data are available on Series record

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