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Series GSE51644 Query DataSets for GSE51644
Status Public on Aug 01, 2014
Title Microarray analysis of gene expression profiles in Map2k1+/-Map2k2+/- and Map2k1flox/-Map2k2+/-Tg+/Sox2Cre placentas
Organism Mus musculus
Experiment type Expression profiling by array
Summary The mammalian genome contains two ERK/MAP kinase kinase genes, Map2k1 and Map2k2, encoding dual-specificity kinases responsible for ERK/MAP kinase activation. Loss of Map2k1 function in mouse causes embryonic lethality due to placental defects, while Map2k2 mutant mice survive with a normal lifespan. The Map2k1 mutation interferes with the growth of the labyrinthine region of the placenta and its vascularization. Map2k1+/-Map2k2+/- embryos also die during gestation of underdevelopment of the labyrinth. In Map2k1+/-Map2k2+/- mutants, the vascularization of the labyrinth is reduced and defects in SynT-II formation lead to the accumulation of multinucleated trophoblasts giant cells (MTG). Deletion of both Map2k1 alleles in allantoïs-derived tissues in Map2k1+/ Map2k2+/- placenta (Map2k1flox/-Map2k2+/-Tg+/Sox2Cre) increases the penetrance and the expressivity of the MTG placental phenotype. Microarray analysis with RNA extracted from placentas of E12.5 wt, Map2k1+/-Map2k2+/- and Map2k1flox/-Map2k2+/-Tg+/Sox2Cre embryos was performed to evaluate the molecular impact of the loss of Map2k alleles on placenta development.
Overall design Total RNA was isolated from placentas of E12.5 Map2k1+/+Map2k2+/+(control), from E12.5 Map2k1+/-Map2k2+/- embryos (experimental) and from E12.5 and Map2k1flox/-Map2k2+/-Tg+/Sox2Cre embryos (experimental). Four specimens were analyzed per genotype.
Contributor(s) Charron J, Nadeau V
Citation(s) 24948605
Submission date Oct 24, 2013
Last update date Jun 14, 2018
Contact name Eric R Paquet
Organization name EPFL
Department Life sciences
Lab Naef lab
Street address Station 15 CH - 1015
City Lausanne
State/province Vaud
ZIP/Postal code 1015
Country Switzerland
Platforms (1)
GPL6885 Illumina MouseRef-8 v2.0 expression beadchip
Samples (12)
GSM1250035 Map2k1+/+Map2k2+/+_rep1
GSM1250036 Map2k1+/+Map2k2+/+_rep2
GSM1250037 Map2k1+/+Map2k2+/+_rep3
BioProject PRJNA224159

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Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE51644_RAW.tar 3.1 Mb (http)(custom) TAR
GSE51644_non-normalized.txt.gz 1.9 Mb (ftp)(http) TXT
Raw data are available on Series record
Processed data included within Sample table

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