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Series GSE51654 Query DataSets for GSE51654
Status Public on Dec 31, 2014
Title Dynamic Changes in E-protein Activity are Essential for Treg cell Development
Organism Mus musculus
Experiment type Expression profiling by array
Summary To gain a molecular view of E-proteins with respect to the development of Foxp3+ T cells, we perform microarray studies that would identify transcription factors that are up-regulated as E-proteins levels fall and and Foxp3 expression rises. We hypothesize that such transcription factors activate the synthesis of key proteins necessary for the development of Foxp3+ cells in the thymus (or in the periphery). Among the possible proteins (negatively regulated by E-protein are those involved in IL-2 signaling, since the latter has been shown to be critical for the development of Foxp3+ cells in the thymus. In the planned studies we will extract mRNA from sorted Foxp3+GFP+ thymocytes from wild type (WT) mice and similar cells from E-protein deficient thymocytes. However, since Foxp3+ thymocytes in WT mice already have reduced E-protein levels, differences between thymocytes from these mice and E-protein deficient mice might be minimal. We therefore will also extract mRNA from Foxp3-negative thymocytes from WT mice whose E-proteins levels have not fallen sufficient low to allow Foxp3 expression and Foxp3-negative thymocytes from E-protein deficient mice whose E-proteins are necessarily low but have not expressed Foxp3 for unrelated reasons.
 
Overall design Isolate mRNA from sorted Foxp3+ and Foxp3- thymocytes from either wild type (WT) mice and from E-protein deficient (E2A and HEB knockout) thymocytes; 2 replicates of each of the 4 conditions
 
Contributor(s) Gao P, Yang JZ, Fuss I, Zhang Q, Myers TG, Gardina PJ, Zhang F, Strober W
Citation(s) 25488982
Submission date Oct 24, 2013
Last update date Jun 14, 2018
Contact name Timothy G Myers
E-mail(s) niaid-mrf-geo@nih.gov
Organization name National Institute of Allergy and Infectious Diseases
Department Research Technologies Branch
Lab Genomic Technologies Section
Street address 50 South Drive, Room 5509
City Bethesda
State/province MD
ZIP/Postal code 20892-8005
Country USA
 
Platforms (1)
GPL6885 Illumina MouseRef-8 v2.0 expression beadchip
Samples (8)
GSM1250152 thymocyte_Foxp3pos_WT_rep1
GSM1250153 thymocyte_Foxp3pos_WT_rep2
GSM1250154 thymocyte_FoxP3neg_WT_rep1
Relations
BioProject PRJNA224255

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE51654_RAW.tar 3.1 Mb (http)(custom) TAR
GSE51654_non-normalized.txt.gz 1.3 Mb (ftp)(http) TXT
Raw data are available on Series record
Processed data included within Sample table

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