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| Status |
Public on Nov 20, 2013 |
| Title |
The effect of imatinib therapy on tumor associated macrophages (TAMs) in human gastrointestinal stromal tumor (GIST) |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
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| Summary |
The gene expression profile of TAMs microbead isolated from freshly obtained human GISTs were compared in tumors that were untreated, responding to imatinib (sensitive), or resistant to imatinib (resistant)
The Affymetrix Human Genome U133A 2.0 platform was used
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| Overall design |
TAMs were microbead isolated from 12 untreated, 5 sensitive, and 4 resistant tumors. Tumors were obtained freshly from the operating room from consenting patients using an IRB approved protocol. Tumors were digested using collagenase and TAMs isolated using microbead negative selection for KIT, CD3, and CD56 followed by positive selection for CD14.
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| Contributor(s) |
Cavnar MJ, Zeng S, Kim TS, Sorenson EC, Ocuin LM, Balachandran VP, Seifert AM, Greer JB, Popow R, Crawley MH, Cohen NA, Green BL, Rossi F, Besmer P, Antonescu CR, DeMatteo RP |
| Citation(s) |
24323358 |
| Submission date |
Oct 25, 2013 |
| Last update date |
Dec 06, 2018 |
| Contact name |
Jeffrey Zhao |
| Organization name |
Memorial Sloan Kettering Cancer Center
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| Department |
Genomics Core
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| Street address |
1275 York Avenue
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| City |
New York |
| State/province |
NY |
| ZIP/Postal code |
10065 |
| Country |
USA |
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| Platforms (1) |
| GPL571 |
[HG-U133A_2] Affymetrix Human Genome U133A 2.0 Array |
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| Samples (21)
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| GSM1250690 |
TAMs sorted from untreated human GIST_HG98 |
| GSM1250691 |
TAMs sorted from untreated human GIST_HG100 |
| GSM1250692 |
TAMs sorted from untreated human GIST_HG101 |
| GSM1250693 |
TAMs sorted from untreated human GIST_HG110 |
| GSM1250694 |
TAMs sorted from untreated human GIST_HG117 |
| GSM1250695 |
TAMs sorted from untreated human GIST_HG120 |
| GSM1250696 |
TAMs sorted from untreated human GIST_HG127 |
| GSM1250697 |
TAMs sorted from untreated human GIST_HG129 |
| GSM1250698 |
TAMs sorted from untreated human GIST_HG132#1 |
| GSM1250699 |
TAMs sorted from untreated human GIST_HG132#2 |
| GSM1250700 |
TAMs sorted from untreated human GIST_HG135 |
| GSM1250701 |
TAMs sorted from untreated human GIST_HG138 |
| GSM1250702 |
TAMs sorted from imatinib sensitive human GIST_HG112 |
| GSM1250703 |
TAMs sorted from imatinib sensitive human GIST_HG114 |
| GSM1250704 |
TAMs sorted from imatinib sensitive human GIST_HG119 |
| GSM1250705 |
TAMs sorted from imatinib sensitive human GIST_HG128 |
| GSM1250706 |
TAMs sorted from imatinib sensitive human GIST_HG133 |
| GSM1250707 |
TAMs sorted from imatinib resistant human GIST_HG106 |
| GSM1250708 |
TAMs sorted from imatinib resistant human GIST_HG108 |
| GSM1250709 |
TAMs sorted from imatinib resistant human GIST_HG113 |
| GSM1250710 |
TAMs sorted from imatinib resistant human GIST_HG130 |
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| Relations |
| BioProject |
PRJNA224583 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE51697_RAW.tar |
42.2 Mb |
(http)(custom) |
TAR (of CEL, CHP) |
| Processed data included within Sample table |
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