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| Status |
Public on Oct 29, 2013 |
| Title |
The gene expression analysis of the spinal cord and skeletal muscle of SBMA (Spinal Bulbar Muscular Atrophy) model mice with or without pioglitazone (PG) treatment |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
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| Summary |
To identify the gene expression changes by administering PG to SBMA model mice, we prepared total RNA samples from the spinal cords and skeletal muscles of transgenic mice carrying a full-length human AR with 97 CAGs (AR-97Q) that were treated with or without PG. We used AR-97Q (Line #7-8) male mice because they show progressive muscular atrophy and weakness as well as SBMA-like pathology such as the accumulation of the pathogenic androgen receptor in the nucleus of motor neurons.
Using microarray analysis, we identified the genes with significantly altered expression of AR-97Q mice by PG treatment.
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| Overall design |
For non-treated and PG-treated groups, we examined the male mice at 13 weeks of age. RNA from the total spinal cord and skeletal muscle was isolated from three mice of each group.
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| Contributor(s) |
Iida M, Katsuno M, Sobue G |
| Citation(s) |
25168383 |
| Submission date |
Oct 28, 2013 |
| Last update date |
Jul 19, 2017 |
| Contact name |
Madoka Iida |
| Organization name |
Nagoya University
|
| Department |
Neurology department
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| Street address |
65 Tsurumai-cho Showa-ku
|
| City |
Nagoya |
| State/province |
Aichi |
| ZIP/Postal code |
466-8550 |
| Country |
Japan |
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| Platforms (1) |
| GPL13912 |
Agilent-028005 SurePrint G3 Mouse GE 8x60K Microarray (Feature Number version) |
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| Samples (12)
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| Relations |
| BioProject |
PRJNA225471 |
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