|
| Status |
Public on Nov 06, 2013 |
| Title |
Low paternal dietary folate alters the sperm epigenome and is associated with negative pregnancy outcomes |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
|
| Summary |
We hypothesized that the availability of folate, a soluble B vitamin, would alter the levels of DNA methylation in spermatogenesis with consequences for the sperm epigenome and pregnancy outcomes. We fed male mice with either a folate-deficient or a folate-sufficient diet throughout life. Males fed the folate-deficient diet had offspring with increased birth defects, which included craniofacial and musculoskeletal malformations. These phenotypes corresponded to developmental genes with altered methylation in sperm. To determine if there was transmission of epigenetic effects from sires to offspring, global gene expression levels were assessed in placenta from 18.5 dpc fetuses sired by either a folate-sufficient or folate-deficient male.
|
| |
|
| Overall design |
Gene expression was measured in placenta of 18.5 dpc fetuses sired by either a folate sufficient male (n=4 placentas from different litters and different fathers) or a folate deficient (FD) male (n=4 placentas from different litters and different fathers).
|
| |
|
| Contributor(s) |
Lambrot R, Kimmins S |
| Citation(s) |
24326934 |
| Submission date |
Oct 28, 2013 |
| Last update date |
Feb 02, 2018 |
| Contact name |
Romain Lambrot |
| E-mail(s) |
romain.lambrot@mcgill.ca
|
| Phone |
5143987663
|
| Organization name |
McGill University
|
| Department |
Animal Science
|
| Lab |
Kimmins Lab
|
| Street address |
21111 Lakeshore road
|
| City |
Ste Anne-de-Bellevue |
| State/province |
QC |
| ZIP/Postal code |
H9X 3V9 |
| Country |
Canada |
| |
|
| Platforms (1) |
| GPL10787 |
Agilent-028005 SurePrint G3 Mouse GE 8x60K Microarray (Probe Name version) |
|
| Samples (8)
|
|
| Relations |
| BioProject |
PRJNA225485 |
Less...
More...