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Series GSE51843 Query DataSets for GSE51843
Status Public on Jan 29, 2014
Title A Pilot Study on the Potential of RNA-Associated to Urinary Vesicles as a Suitable Non-Invasive Source for Diagnostic Purposes in Bladder Cancer
Organism Homo sapiens
Experiment type Expression profiling by array
Summary Abstract: Bladder cancer is one of the most common cancers and, together with prostate carcinoma, accounts for the majority of the malignancies of the genitourinary tract. Since prognosis ameliorates with early detection, it will be beneficial to have a repertoire of diagnostic markers that could complement the current diagnosis protocols. Recently, cell-secreted extracellular vesicles have received great interest as a source of low invasive disease biomarkers because they are found in many body fluids, including urine. The current work describes a pilot study to generate an array-based catalogue of mRNA associated to urinary vesicles, and also a comparison with samples obtained from bladder cancer patients. After an analysis of presence/absence of transcripts in bladder cancer EVs, a list of genes was selected for further validation using PCR technique. We found four genes differentially expressed in cancer samples. LASS2 and GALNT1 were present in cancer patients, while ARHGEF39 and FOXO3 were found only in non-cancer urinary vesicles. Previous studies have pointed to the involvement of those genes in tumour progression and metastasis.
Overall design Total RNA isolated from Extracellular Vesicles obtained from urine of bladder cancer patients was compared with RNA isolated from urinary vesicles of non-cancer patients.
Contributor(s) Perez A, Loizaga A, Arceo R, Lacasa I, Rabade A, Zorroza K, Mosen-Ansorena D, Gonzalez E, Aransay AM, Falcon-Perez JM, Unda-Urzaiz M, Royo F
Citation(s) 24458310
Submission date Oct 29, 2013
Last update date Aug 13, 2018
Contact name Ana Maria Aransay
Phone 34944061325
Organization name CIC bioGUNE
Department Genome Analysis Platform
Street address Parque tecnologico de bizkaia, Building 801-A
City Derio
State/province BIZKAIA
ZIP/Postal code 48160
Country Spain
Platforms (1)
GPL10558 Illumina HumanHT-12 V4.0 expression beadchip
Samples (11)
GSM1253682 Control 1
GSM1253683 Control 2
GSM1253684 Control 3
BioProject PRJNA225592

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE51843_RAW.tar 26.2 Mb (http)(custom) TAR
GSE51843_non-normalized.txt.gz 2.1 Mb (ftp)(http) TXT
Raw data are available on Series record
Processed data included within Sample table

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