This data was used to identify miRNA signatures that can be used to calssify specific leukemia types including AML, precursor B ALL, precursor T ALL, and normal hematopoietic progenitor and mature populations This data was used to identify miRNA signatures that can be used to calssify specific leukemia types including AML, precursor B ALL, precursor T ALL, and normal hematopoietic progenitor and mature populations
Overall design
AML cell lines and patient samples, B ALL cell lines and Patient samplest, T ALL cell lines and patient samples, norma B cells, normal granulocytes, normal monocytes, normal T cells and normal CD34+ cells were used for RNA extraction and hybridization on Affymetrix microarrays. All the AML, B ALL, T ALL cell lines were cultured in vitro under appropriate culture conditons and harvested in their log phase growth for RNA extraction. AML, B ALL, and T ALL patient samples were collected...(I assume these are the PBMCs from eitehr peripheral blood or bone marrow from patients, please confirm). Normal B cells, granulocytes, monocytes, and T cells were purified from human peripheral blood of normal healthy donors.
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