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| Status |
Public on Dec 01, 2015 |
| Title |
Microarray expression analysis in idiopathic and LRRK2-associated Parkinson's disease (PD) |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
|
| Summary |
We analysed the RNA profile of IPSC-derived dopaminergic neurons from idiophatic and genetic form (LRRK2) of Parkinson’s disease (PD). Both, idiopathic and genetic form of the disease show similar expression alterations and were merged in one whole PD group. We found 437 differentially expressed genes (DEGs) in the PD group as a whole. Up-regulated DEGs (n=254) encompassed genes involved in neural functions and transcription factor functions whereas down-regulated DEGs (n=183) affected basic homeostasis. These data point towards the presence of gene - and also protein - expression changes in DAn from PD patients which co-occur simultaneously along with DNA methylation changes.
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| Overall design |
Number of samples analyzed: 14 iPSC-derived dopaminergic neurons . Control (C) samples, no pathological changes: SP9, SP11 SP15 SP17. Samples from idiopathic Parkinson's Disease (IPD) patients: SP1, SP2, SP4, SP8, SP10, SP16. Samples from Parkinson's disease patients carriers of G2019S LRRK2 mutation (MPD): SP5, SP6, SP12, SP13. No replicates. 30 days of cell culture.
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| Contributor(s) |
Ezquerra M, Fernandez-Santiago R, Tolosa E, Sànchez-Pla À, Mosquera JL |
| Citation(s) |
26516212 |
| Submission date |
Oct 30, 2013 |
| Last update date |
Feb 18, 2019 |
| Contact name |
Mario Ezquerra |
| E-mail(s) |
ezquerra@clinic.cat
|
| Phone |
0034 932273122
|
| Organization name |
Fundacio Clinic/IDIBAPS
|
| Department |
Neurology
|
| Lab |
Neurodegenerative Diseases
|
| Street address |
Villarroel 170
|
| City |
Barcelona |
| State/province |
Catalonia |
| ZIP/Postal code |
08027 |
| Country |
Spain |
| |
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| Platforms (1) |
| GPL5175 |
[HuEx-1_0-st] Affymetrix Human Exon 1.0 ST Array [transcript (gene) version] |
|
| Samples (14)
|
| GSM1255324 |
RNA-30 days-neuron-control4 |
| GSM1255325 |
RNA-30 days-neuron-idiopathic PD patient1 |
| GSM1255326 |
RNA-30 days-neuron-idiopathic PD patient2 |
| GSM1255327 |
RNA-30 days-neuron-idiopathic PD patient3 |
| GSM1255328 |
RNA-30 days-neuron-idiopathic PD patient4 |
| GSM1255329 |
RNA-30 days-neuron-idiopathic PD patient5 |
| GSM1255330 |
RNA-30 days-neuron-idiopathic PD patient6 |
| GSM1255331 |
RNA-30 days-neuron-PD patient LRRK2 G2019S mutation carrier 1 |
| GSM1255332 |
RNA-30 days-neuron-PD patient LRRK2 G2019S mutation carrier 2 |
| GSM1255333 |
RNA-30 days-neuron-PD patient LRRK2 G2019S mutation carrier 3 |
| GSM1255334 |
RNA-30 days-neuron-PD patient LRRK2 G2019S mutation carrier 4 |
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| This SubSeries is part of SuperSeries: |
| GSE51923 |
Idiopathic and LRRK2-associated Parkinson's disease |
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| Relations |
| BioProject |
PRJNA225814 |
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