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| Status |
Public on Jul 07, 2006 |
| Title |
Transcriptional profiling of mouse ileum in response to colonization with a zebrafish or mouse gut microbiota |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
|
| Summary |
We compared gene expression in the small intestine (ileum) of mice that were either (i) germ-free, (ii) colonized with a conventional mouse cecal microbiota, (iii) colonized with a conventional zebrafish gut microbiota, or (iv) colonized with Pseudomonas aeruginosa PAO1.
Keywords: response to microbial colonization
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| |
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| Overall design |
Adult germ-free NMRI mice were colonized with either (i) a conventional mouse cecal microbiota harvested from adult Swiss-Webster mice (5 biological replicates), (ii) a conventional zebrafish intestinal microbiota harvested from adult C32 zebrafish (3 biological replicates), or (iii) a culture of Pseudomonas aeruginosa PAO1 (5 biological replicates). 14 days after colonization, total RNA was prepared from the ileum of each animal, with total RNA prepared from adult germ-free NMRI mouse ileum serving as negative controls (5 biological replicates). RNA was used as template to generate cRNA for hybridization to Affymetrix 430 v2 Mouse GeneChips.
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| Contributor(s) |
Rawls JF, Mahowald MA, Ley RE, Gordon JI |
| Citation(s) |
17055441 |
| Submission date |
Jun 29, 2006 |
| Last update date |
Feb 11, 2019 |
| Contact name |
John F Rawls |
| E-mail(s) |
jfrawls@med.unc.edu
|
| Phone |
919-966-8340
|
| Fax |
919 966-6927
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| Organization name |
University of North Carolina at Chapel Hill
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| Department |
Department of Cell and Molecular Physiology
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| Street address |
111 Mason Farm Rd.
|
| City |
Chapel Hill |
| State/province |
NC |
| ZIP/Postal code |
27599-7545 |
| Country |
USA |
| |
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| Platforms (1) |
| GPL1261 |
[Mouse430_2] Affymetrix Mouse Genome 430 2.0 Array |
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| Samples (18)
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| GSM116746 |
Ileum_Germ-free_rep4_9774 |
| GSM116747 |
Ileum_Germ-free_rep5_9775 |
| GSM117089 |
Ileum_PA01-colonized_rep1_9776 |
| GSM117090 |
Ileum_PA01-colonized_rep2_9777 |
| GSM117096 |
Ileum_PA01-colonized_rep3_9778 |
| GSM117110 |
Ileum_PA01-colonized_rep4_9779 |
| GSM117119 |
Ileum_PA01-colonized_rep5_9780 |
| GSM117161 |
Ileum_Mouse-microbiota-colonized_rep1_9786 |
| GSM117164 |
Ileum_Mouse-microbiota-colonized_rep2_9787 |
| GSM117165 |
Ileum_Mouse-microbiota-colonized_rep3_9788 |
| GSM117173 |
Ileum_Mouse-microbiota-colonized_rep4_9789 |
| GSM117180 |
Ileum_Mouse-microbiota-colonized_rep5_9790 |
| GSM117189 |
Ileum_Zebrafish-microbiota-colonized_rep1_9791 |
| GSM117197 |
Ileum_Zebrafish-microbiota-colonized_rep2_9792 |
| GSM117205 |
Ileum_Zebrafish-microbiota-colonized_rep3_9793 |
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| Relations |
| BioProject |
PRJNA96557 |
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