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Series GSE5198 Query DataSets for GSE5198
Status Public on Jul 07, 2006
Title Transcriptional profiling of mouse ileum in response to colonization with a zebrafish or mouse gut microbiota
Organism Mus musculus
Experiment type Expression profiling by array
Summary We compared gene expression in the small intestine (ileum) of mice that were either (i) germ-free, (ii) colonized with a conventional mouse cecal microbiota, (iii) colonized with a conventional zebrafish gut microbiota, or (iv) colonized with Pseudomonas aeruginosa PAO1.
Keywords: response to microbial colonization
Overall design Adult germ-free NMRI mice were colonized with either (i) a conventional mouse cecal microbiota harvested from adult Swiss-Webster mice (5 biological replicates), (ii) a conventional zebrafish intestinal microbiota harvested from adult C32 zebrafish (3 biological replicates), or (iii) a culture of Pseudomonas aeruginosa PAO1 (5 biological replicates). 14 days after colonization, total RNA was prepared from the ileum of each animal, with total RNA prepared from adult germ-free NMRI mouse ileum serving as negative controls (5 biological replicates). RNA was used as template to generate cRNA for hybridization to Affymetrix 430 v2 Mouse GeneChips.
Contributor(s) Rawls JF, Mahowald MA, Ley RE, Gordon JI
Citation(s) 17055441
Submission date Jun 29, 2006
Last update date Feb 11, 2019
Contact name John F Rawls
Phone 919-966-8340
Fax 919 966-6927
Organization name University of North Carolina at Chapel Hill
Department Department of Cell and Molecular Physiology
Street address 111 Mason Farm Rd.
City Chapel Hill
State/province NC
ZIP/Postal code 27599-7545
Country USA
Platforms (1)
GPL1261 [Mouse430_2] Affymetrix Mouse Genome 430 2.0 Array
Samples (18)
GSM116743 Ileum_Germ-free_rep1_9771
GSM116744 Ileum_Germ-free_rep2_9772
GSM116745 Ileum_Germ-free_rep3_9773
BioProject PRJNA96557

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Supplementary file Size Download File type/resource
GSE5198_RAW.tar 113.6 Mb (http)(custom) TAR (of CEL)
Raw data provided as supplementary file

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