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| Status |
Public on Jun 30, 2006 |
| Title |
Expression data from B. melitensis infected mouse macrophages |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
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| Summary |
Facultative intracellular Brucella infect and survive inside macrophages, and the outcome of macrophage-Brucella interaction is a basis for establishment of a chronic Brucella infection. The majority of Brucella are killed at the early infection stage. A subpopulation of virulent Brucella strains is instead trafficked to an intracellular replicative phagosome, and are resistant to further attack and begin to multiply dramatically. Virulent Brucella also inhibit macrophage apoptosis that in turn favors pathogen survival and replication.
We used the Affymetrix mouse GeneChip 430 2.0 array to analyze mouse macrophage gene expression profiles during the time course of virulent B. melitensis strain 16M infection.
Keywords: time course
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| Overall design |
Murine J774.A1 macrophage cells were infected with B. melitensis strain 16M at a MOI of 200:1. Brucella cultures derived from different Brucella colonies were used to infect different groups of macrophage cells to reflect independent infections. Following 4 h incubation, the cells were washed with PBS and treated with 50 ug/ml gentamicin to kill extracellular Brucella. At 0 h (no Brucella infection), 4 h, 24 h, and 48 h post-infection, cells were individually collected, and total RNA was isolated. The Affymetrix mouse GeneChip 430 2.0 array was used for microarray hybridization. Microarray intensity data were obtained by using Affymetrix GCOS software and further analyzed by GeneSpring and other software programs.
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| Contributor(s) |
He Y, Reichow S, Ramamoorthy S, Ding X, Sriranganathan N, Boyle S |
| Citation(s) |
16926395 |
| Submission date |
Jun 30, 2006 |
| Last update date |
Aug 02, 2019 |
| Contact name |
Yongqun He |
| E-mail(s) |
yongqunh@umich.edu
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| Phone |
734-615-8231
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| Fax |
734-936-3235
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| URL |
http://www.hegroup.org/
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| Organization name |
University of Michigan
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| Street address |
018 ARF, 1150 W. Medical Center Dr.
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| City |
Ann Arbor |
| State/province |
MI |
| ZIP/Postal code |
48109 |
| Country |
USA |
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| Platforms (1) |
| GPL1261 |
[Mouse430_2] Affymetrix Mouse Genome 430 2.0 Array |
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| Samples (12)
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| GSM117560 |
macrophages at 0h post infection with B. melitensis strain 16M, biological rep1 |
| GSM117561 |
macrophages at 0h post infection with B. melitensis strain 16M, biological rep2 |
| GSM117562 |
macrophages at 0h post infection with B. melitensis strain 16M, biological rep3 |
| GSM117563 |
macrophages at 4 h post infection with B. melitensis strain 16M, biological rep1 |
| GSM117564 |
macrophages at 4 h post infection with B. melitensis strain 16M, biological rep2 |
| GSM117565 |
macrophages at 4 h post infection with B. melitensis strain 16M, biological rep3 |
| GSM117566 |
macrophages at 24h post infection with B. melitensis strain 16M, biological rep1 |
| GSM117567 |
macrophages at 24 h post infection with B. melitensis strain 16M, biological rep2 |
| GSM117568 |
macrophages at 24 h post infection with B. melitensis strain 16M, biological rep3 |
| GSM117569 |
macrophages at 48 h post infection with B. melitensis strain 16M, biological rep1 |
| GSM117570 |
macrophages at 48 h post infection with B. melitensis strain 16M, biological rep2 |
| GSM117571 |
macrophages at 48 h post infection with B. melitensis strain 16M, biological rep3 |
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| Relations |
| BioProject |
PRJNA96565 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE5202_RAW.tar |
41.4 Mb |
(http)(custom) |
TAR (of CEL) |
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