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Series GSE5202 Query DataSets for GSE5202
Status Public on Jun 30, 2006
Title Expression data from B. melitensis infected mouse macrophages
Organism Mus musculus
Experiment type Expression profiling by array
Summary Facultative intracellular Brucella infect and survive inside macrophages, and the outcome of macrophage-Brucella interaction is a basis for establishment of a chronic Brucella infection. The majority of Brucella are killed at the early infection stage. A subpopulation of virulent Brucella strains is instead trafficked to an intracellular replicative phagosome, and are resistant to further attack and begin to multiply dramatically. Virulent Brucella also inhibit macrophage apoptosis that in turn favors pathogen survival and replication.
We used the Affymetrix mouse GeneChip 430 2.0 array to analyze mouse macrophage gene expression profiles during the time course of virulent B. melitensis strain 16M infection.
Keywords: time course
Overall design Murine J774.A1 macrophage cells were infected with B. melitensis strain 16M at a MOI of 200:1. Brucella cultures derived from different Brucella colonies were used to infect different groups of macrophage cells to reflect independent infections. Following 4 h incubation, the cells were washed with PBS and treated with 50 ug/ml gentamicin to kill extracellular Brucella. At 0 h (no Brucella infection), 4 h, 24 h, and 48 h post-infection, cells were individually collected, and total RNA was isolated. The Affymetrix mouse GeneChip 430 2.0 array was used for microarray hybridization. Microarray intensity data were obtained by using Affymetrix GCOS software and further analyzed by GeneSpring and other software programs.
Contributor(s) He Y, Reichow S, Ramamoorthy S, Ding X, Sriranganathan N, Boyle S
Citation(s) 16926395
Submission date Jun 30, 2006
Last update date Aug 02, 2019
Contact name Yongqun He
Phone 734-615-8231
Fax 734-936-3235
Organization name University of Michigan
Street address 018 ARF, 1150 W. Medical Center Dr.
City Ann Arbor
State/province MI
ZIP/Postal code 48109
Country USA
Platforms (1)
GPL1261 [Mouse430_2] Affymetrix Mouse Genome 430 2.0 Array
Samples (12)
GSM117560 macrophages at 0h post infection with B. melitensis strain 16M, biological rep1
GSM117561 macrophages at 0h post infection with B. melitensis strain 16M, biological rep2
GSM117562 macrophages at 0h post infection with B. melitensis strain 16M, biological rep3
BioProject PRJNA96565

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Supplementary file Size Download File type/resource
GSE5202_RAW.tar 41.4 Mb (http)(custom) TAR (of CEL)
Raw data provided as supplementary file

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