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| Status |
Public on Nov 01, 2020 |
| Title |
A low meal frequency improves 24h metabolic and gene expression profiles in subjects with impaired glucose tolerance (PBMC) |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
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| Summary |
Meal frequency regulates postprandial glucose and insulin responses, which may affect substrate partitioning and thus weight control. This study investigated the effects of meal frequency on the metabolic and transcriptomic profiles in subjects with impaired glucose tolerance (IGT). Eleven IGT (2h glucose; 9.0±0.3mmol/L) men stayed 2x 36 hours in a respiration chamber to measure substrate partitioning. All subjects randomly received two isoenergetic diets with a low meal frequency (3x-LFr) or a high meal frequency (14x-HFr) consisting of 15 En% prot, 30 En% fat, and 55 En% carb. Total glucose output (AUC), carbohydrate oxidation and whole body RQ significantly decreased, and FFA levels increased in the LFr diet. The HFr diet resulted in an up-regulation in expression of genes involved in immune function and inflammation after 24h in PBMCs and muscle tissue. Expression of genes involved in PPAR signalling, OXPHOS, and glutathione metabolism were up-regulated in the HFr diet in muscle tissue only. In conclusion, the LFr diet improved the metabolic and transcriptomic profiles, and appetite control compared to the HFr diet. This suggests that a LFr diet might be an effective dietary strategy with anti-inflammatory characteristics to increase metabolic flexibility and body weight control in IGT subjects.
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| Overall design |
In a randomized, 2-way crossover study, 11 men with impaired glucose tolerance stayed 2x 36 hours in a respiration chamber to measure the effect of meal frequency on substrate partitioning and gene expression in peripheral blood mononuclear cells (PBMCs) and muscle tissue. This entry contains the PBMC expression data.
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| Contributor(s) |
Munsters MJ, Boekschoten MV, Vijgen G, Afman LA, Saris WH |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| Submission date |
Nov 03, 2013 |
| Last update date |
Nov 01, 2020 |
| Contact name |
Guido Hooiveld |
| E-mail(s) |
guido.hooiveld@wur.nl
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| Organization name |
Wageningen University
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| Department |
Div. Human Nutrition & Health
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| Lab |
Nutrition, Metabolism & Genomics Group
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| Street address |
HELIX, Stippeneng 4
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| City |
Wageningen |
| ZIP/Postal code |
NL-6708WE |
| Country |
Netherlands |
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| Platforms (1) |
| GPL11532 |
[HuGene-1_1-st] Affymetrix Human Gene 1.1 ST Array [transcript (gene) version] |
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| Samples (61)
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| This SubSeries is part of SuperSeries: |
| GSE52028 |
A low meal frequency improves 24h metabolic and gene expression profiles in subjects with impaired glucose tolerance |
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| Relations |
| BioProject |
PRJNA226235 |
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