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Series GSE5213 Query DataSets for GSE5213
Status Public on Aug 01, 2006
Title Signature of human hepatocytes treated with Aroclor1254
Organism Homo sapiens
Experiment type Expression profiling by array
Summary In order to determine molecular rules for transcriptional regulation of targeted genes to explain in part the pleiotropic effect observed in animals and humans upon exposure to Aroclor 1254 we treated human hepatocytes with Aroclor1254 and analysed RNA expression using a Nimblegene human custom array.
Keywords: Human hepatocytes, Aroclor1254 treatment, 20µM, 72h
 
Overall design Human hepatocytes isolated from surgical material were treated with 20µM Aroclor1254 for 72h. Non-treated cells served as control. Total RNA was isolated using QIAGEN´s RNeasy total RNA isolation kit according to the manufacturer’s recommendations. Oligonucleotide microarrays were hybridized and washed according to the manufacturer’s recommendations.
 
Citation(s) 16934159
Submission date Jul 03, 2006
Last update date Mar 16, 2012
Contact name Jürgen Borlak
E-mail(s) borlak@item.fraunhofer.de
Phone +49-511 5350-520
Fax +49-511 5350-573
URL http://www.item.fraunhofer.de
Organization name Fraunhofer Institut
Street address Nikolai-Fuchs-Strasse 1
City Hannover
ZIP/Postal code 30625
Country Germany
 
Platforms (1)
GPL3872 Nimblegen Human custom array
Samples (2)
GSM114140 Control
GSM114319 Aroclor1254 treatment, 20µM, 72h
Relations
BioProject PRJNA96507

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