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GEO help: Mouse over screen elements for information. |
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| Status |
Public on Jul 08, 2006 |
| Title |
Cross-regulation between Notch and p63 in keratinocyte commitment to differentiation |
| Organisms |
Homo sapiens; Mus musculus |
| Experiment type |
Expression profiling by array
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| Summary |
Notch signaling promotes commitment of keratinocytes to differentiation and suppresses tumorigenesis. p63, a p53 family member, has been implicated in establishment of the keratinocyte cell fate and/or maintenance of epithelial self-renewal. Here we show that p63 expression is suppressed by Notch1 activation in both mouse and human keratinocytes through a mechanism independent of cell cycle withdrawal and requiring down-modulation of selected interferon-responsive genes, including IRF7 and/or IRF3. In turn, elevated p63 expression counteracts the ability of Notch1 to restrict growth and promote differentiation. p63 functions as a selective modulator of Notch1-dependent transcription and function, with the Hes-1 gene as one of its direct negative targets. Thus, a complex cross-talk between Notch and p63 is involved in the balance between keratinocyte self-renewal and differentiation.
Keywords: Notch1, p63, keratinocyte differentiation, gene expression profiling
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| Overall design |
PolyA+ mRNA (2-5 ug) from cells infected with the Ad GFP and Ad-NotchIC viruses was used as template for double stranded cDNA preparations with a T7-(dT)24 oligonucleotide primers for the first strand reaction. The resulting cDNA was used for preparation of biotin-labeled antisense cRNA and further used for hybridization to Affymetrix U74A or U95A “gene chips” according to the manufacturer’s recommendation.
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| Contributor(s) |
Nguyen BC, Wang J, Devgan V, Lefort K, Buck C, Antonini D, Kitajewski J, Chiorino G, Missero C, Dotto GP |
| Citation(s) |
16618808 |
| Submission date |
Jul 05, 2006 |
| Last update date |
Jan 08, 2019 |
| Contact name |
G.Paolo Dotto |
| E-mail(s) |
gdotto@partners.org
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| Phone |
011-41-21-692 5851
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| Organization name |
Lausanne University
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| Department |
Biochemistry
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| Lab |
Dotto
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| Street address |
153 Chemin de Bovaress
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| City |
Epalinges |
| State/province |
VD |
| ZIP/Postal code |
1066 |
| Country |
Switzerland |
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| Platforms (2) |
| GPL339 |
[MOE430A] Affymetrix Mouse Expression 430A Array |
| GPL571 |
[HG-U133A_2] Affymetrix Human Genome U133A 2.0 Array |
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| Samples (4)
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| Relations |
| BioProject |
PRJNA96431 |
| Supplementary data files not provided |
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