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| Status |
Public on Jul 05, 2006 |
| Title |
Expression data from Lbeta T2 cells upon activin treatment |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
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| Summary |
The primary response of Lbeta T 2cells upon activin treatment at 1hr, 2hr, and 6hr is the significant up-regulation of FSHbeta subunit. Affymetrix genearray missed this important information due to cross-hybridization of its probes with other genes and chromosome segments.
Activin modulates a network of genes and signaling pathways to exert a variety of functions.
Keywords: time course
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| Overall design |
Subconfluent Lbeta T2 cells were seeded in cell culture flasks for 16 hours in phenol-free, serum-free medium, and then treated with either vehicle or activin A (30ng/ml). At 1hr, 2hr, or 6hr after treatment, total RNA was isolated using RNeasy Mini Kit. Integrity of RNA was evaluated with an Agilent 2100 Bioanalyzer, and purity/concentration was determined using a GeneSpec III before target preparationi and hybridizations.
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| Contributor(s) |
Peng Y, Li X, Woodruff T |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| Submission date |
Jul 05, 2006 |
| Last update date |
Feb 11, 2019 |
| Contact name |
Ying Peng |
| E-mail(s) |
yingp@northwestern.edu
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| Organization name |
Northwestern University
|
| Street address |
2205 Tech Drive
|
| City |
Evanston |
| State/province |
IL |
| ZIP/Postal code |
60208 |
| Country |
USA |
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| Platforms (1) |
| GPL1261 |
[Mouse430_2] Affymetrix Mouse Genome 430 2.0 Array |
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| Samples (6)
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| Relations |
| BioProject |
PRJNA96437 |
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