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Series GSE52397 Query DataSets for GSE52397
Status Public on Nov 17, 2013
Title C/EBPα poises B cells for rapid reprogramming into iPS cells
Organism Mus musculus
Experiment type Expression profiling by array
Genome binding/occupancy profiling by high throughput sequencing
Expression profiling by high throughput sequencing
Summary C/EBPα induces transdifferentiation of B cells into macrophages at high efficiencies and enhances reprogramming into induced pluripotent stem cells (iPSCs) when co-expressed with Oct4, Sox2, Klf4 and Myc (OSKM). However, how C/EBPα accomplishes these effects is unclear. We now found that transient C/EBPα expression followed by OSKM activation induces a 100 fold increase in iPSC reprogramming efficiency, involving 95% of the cells. During this conversion pluripotency and epithelial-mesenchymal transition genes become dramatically up-regulated and 60% of the cells express Oct4 within 2 days. C/EBPα acts as a pathbreaker since it transiently makes the chromatin of pluripotency genes more accessible to DNase I. It also induces the expression of the dioxygenase Tet2 and promotes its translocation to the nucleus where it binds to regulatory regions of pluripotency genes that become demethylated following OSKM induction. In line with these findings, overexpression of Tet2 enhances OSKM‐induced B cell reprogramming. Since the enzyme is also required for efficient C/EBPα-induced immune cell conversion, our data suggest that Tet2 provides a mechanistic link between iPSC reprogramming and B cell transdifferentiation. The rapid iPS reprogramming approach described should help to fully elucidate the process and has potential clinical applications.
 
Overall design Refer to individual Series
 
Citation(s) 24336202, 25977766
Submission date Nov 14, 2013
Last update date Jul 31, 2019
Contact name Bruno Di Stefano
E-mail(s) distefanob@gmail.com
Organization name Harvard University
Department Harvard Dept. of Stem Cell and Regenerative Biology
Street address 185 Cambridge Street
City Boston
State/province Ma
ZIP/Postal code 02114
Country USA
 
Platforms (2)
GPL13112 Illumina HiSeq 2000 (Mus musculus)
GPL13912 Agilent-028005 SurePrint G3 Mouse GE 8x60K Microarray (Feature Number version)
Samples (48)
GSM1128619 18hrs pulse B cells, time 0, technical replicate 1
GSM1128620 18hrs pulse B cells, time 0, technical replicate 2
GSM1128621 18hrs pulse B cells, time 48, technical replicate 1
This SuperSeries is composed of the following SubSeries:
GSE46321 C/EBPα poises B cells for rapid reprogramming into iPS cells [array]
GSE52373 C/EBPα poises B cells for rapid reprogramming into iPS cells [ChIP-Seq]
GSE52396 C/EBPα poises B cells for rapid reprogramming into iPS cells [RNA-Seq]
Relations
BioProject PRJNA227776

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE52397_RAW.tar 528.9 Mb (http)(custom) TAR (of BED, TSV, TXT)
SRA Run SelectorHelp
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