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Series GSE52424 Query DataSets for GSE52424
Status Public on Jan 01, 2014
Title Gene expression from Prep1-ablated mice
Organism Mus musculus
Experiment type Expression profiling by array
Summary The homeodomain transcription factor Prep1 was previously shown to regulate insulin sensitivity. Our aim was to study the specific role of Prep1 for the regulation of energy metabolism in skeletal muscle. Muscle specific ablation of Prep1 resulted in increased expression of respiratory chain subunits. This finding was consistent with an increase in mitochondrial enzyme activity without affecting mitochondrial volume fraction as assessed by electron microscopy. Metabolic phenotyping revealed no differences in daily energy expenditure or body composition. However, during treadmill exercise challenge, Prep1 ablation resulted in a higher maximal oxidative capacity and better endurance. Elevated PGC-1α expression was identified as a cause for increased mitochondrial capacity in Prep1-ablated mice. Prep1 stabilizes p160 Mybbp1a, a known inhibitor of PGC-1α activity. Thereby, P160 protein levels were significantly lower in muscle of Prep1-ablated mice. By a ChIPseq approach, PREP1-binding sites in genes encoding mitochondrial components (e.g. Ndufs2) were identified that might be responsible for elevated OXPHOS proteins in the muscle of Prep1 nullmutants. These results suggest that Prep1 exhibits additional direct effects on regulation of mitochondrial proteins. We therefore conclude that Prep1 is a regulator of oxidative phosphorylation components via direct and indirect mechanisms.
 
Overall design Consequence of Prep1 ablation in skeletal muscle was investigated in Prep1deltaSM mice and compared to Prep1 flox mice, both on C57BL/6 background. 4 mice of each genotype were used to extract RNA from the tibialis anterior muscle.
 
Contributor(s) Kanzleiter T
Citation(s) 24216763
Submission date Nov 15, 2013
Last update date Jan 12, 2017
Contact name Timo Kanzleiter
Organization name DIfE
Department Experimental Diabetology
Street address Arthur-Scheunert-Alle 114
City Nuthetal
ZIP/Postal code 14558
Country Germany
 
Platforms (1)
GPL7202 Agilent-014868 Whole Mouse Genome Microarray 4x44K G4122F (Probe Name version)
Samples (8)
GSM1266595 Prep1f/+_P286
GSM1266596 Prep1f/+_P62
GSM1266597 Prep1f/+_P66
Relations
BioProject PRJNA228911

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Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE52424_RAW.tar 67.4 Mb (http)(custom) TAR (of TXT)
Raw data provided as supplementary file
Processed data included within Sample table

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