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Series GSE52509 Query DataSets for GSE52509
Status Public on Dec 04, 2014
Title Cigarette smoke-induced iBALT mediates macrophage activation in a B cell-dependent manner in COPD
Organism Mus musculus
Experiment type Expression profiling by array
Summary Chronic obstructive pulmonary disease (COPD) is characterized by a progressive decline in lung function, caused by exposure to exogenous particles, mainly cigarette smoke (CS). COPD pathogenesis is initiated and perpetuated by an abnormal CS-induced inflammatory response of the lungs, involving both innate and adaptive immunity. Specifically, B cells organized in iBALT structures, as well as macrophages, accumulate in the lungs and contribute to CS-induced emphysema, but the mechanisms thereof remain unclear.
Here, we demonstrate that B cell-deficient mice are significantly protected against CS-induced emphysema. Chronic CS exposure led to increased lung compliance, total lung capacity, and mean linear chord length in WT, but not B cell-deficient mice, associated with an increased size and number of iBALT structures. The increased accumulation of macrophages around iBALT and in emphysematous alveolar areas in CS-exposed WT mice coincided with upregulated MMP12 expression. In vitro co-culture experiments using B cells and macrophages demonstrated that B cell-derived IL-10 drives macrophage activation and MMP12 upregulation. In summary, B cell function in iBALT formation in CS-induced emphysema provides a new innovative mechanism, which could be explored as a target for therapeutic intervention in COPD patients.
 
Overall design Expression data of mice treated with cigarette smoke. Lung tissue was analysed at four and six months of age.
 
Contributor(s) John-Schuster G, Hager K, Irmler M, Beckers J, Conlon TM, Eickelberg O, Yildirim AO
Citation(s) 25128521
Submission date Nov 19, 2013
Last update date Jun 14, 2018
Contact name Johannes Beckers
E-mail(s) beckers@helmholtz-muenchen.de
Organization name Helmholtz Zentrum Muenchen
Department Institute of Experimental Genetics
Street address Ingolstaedter Landstr. 1
City Neuherberg
ZIP/Postal code 85764
Country Germany
 
Platforms (1)
GPL6885 Illumina MouseRef-8 v2.0 expression beadchip
Samples (12)
GSM1267994 Lung tissue from cigarette smoke-treated mice at 4 months of age, biological replicate 1
GSM1267995 Lung tissue from cigarette smoke-treated mice at 4 months of age, biological replicate 2
GSM1267996 Lung tissue from cigarette smoke-treated mice at 4 months of age, biological replicate 3
Relations
BioProject PRJNA229140

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE52509_RAW.tar 3.1 Mb (http)(custom) TAR
GSE52509_non-normalized.txt.gz 947.8 Kb (ftp)(http) TXT
Raw data are available on Series record
Processed data included within Sample table

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