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Series GSE52522 Query DataSets for GSE52522
Status Public on Nov 20, 2013
Title Transcriptional program for induced delta cell on day10
Organism Mus musculus
Experiment type Expression profiling by array
Summary Direct lineage conversion of adult cells is a promising approach for regenerative medicine. A major challenge of lineage conversion is to generate specific subtypes of cells, closely related cells with distinct properties. The pancreatic islets contain three major hormone-secreting endocrine subtypes: insulin+ β-cells, glucagon+ α-cells, and somatostatin+ δ-cells. We previously reported that a combination of three transcription factors, Ngn3, Mafa, and Pdx1, directly reprogram pancreatic acinar cells to β-cells. We now show that acinar cells can be converted to δ-like and α-like cells by Ngn3 and Ngn3+Mafa respectively. Thus, three major islet endocrine subtypes can be derived by acinar reprogramming. Ngn3 promotes establishment of a generic endocrine state in acinar cells at the onset of reprogramming in addition to promoting δ-specification. Mafa and Pdx1 suppress δ-specification in α- and β-cell formation. These studies identify a set of defined factors whose combinatorial actions reprogram acinar cells to distinct islet endocrine subtypes in vivo.
 
Overall design induced beta cells samples at day 10 collected for the microarray
 
Contributor(s) Li W, Zhou Q
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Submission date Nov 19, 2013
Last update date Jun 14, 2018
Contact name Weida Li
E-mail(s) weidali@mcb.harvard.edu
Phone 6174961687
Organization name Harvard University
Street address 7 Divninity Avenue
City Cambridge
State/province MA
ZIP/Postal code 02138
Country USA
 
Platforms (1)
GPL6885 Illumina MouseRef-8 v2.0 expression beadchip
Samples (3)
GSM1268705 induced delta 1
GSM1268706 induced delta 2
GSM1268707 induced delta 3
Relations
BioProject PRJNA229154

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE52522_RAW.tar 3.1 Mb (http)(custom) TAR
GSE52522_non-normalized.txt.gz 609.9 Kb (ftp)(http) TXT
Processed data included within Sample table
Raw data are available on Series record
Processed data are available on Series record

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