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Series GSE52766 Query DataSets for GSE52766
Status Public on Oct 13, 2014
Title The effects of muscular dystrophy, exercise and sActRIIB-Fc on molecular signature of skeletal muscle in mice
Organism Mus musculus
Experiment type Expression profiling by array
Summary To study the combined effect of myostatin/activin inhibition and exercise on muscle mass and pathophysiology, young mdx mice, a model for Duchenne Muscular Dystrophy, were injected with soluble activin receptor-Fc (sActRIIB-Fc) or placebo (PBS) 1x/week for a 7-week period, in combination with or without voluntary running. C57Bl/10ScSnJ mice injected with PBS acted as wildtype controls. Microarray expression analysis from skeletal muscle was performed using m. gastrocnemies as the sample.
We found thatexercise or a combination of exercise and sActRIIB-Fc treatment is more effective in correcting gene expression profiles of dystrophic muscles than the sActRIIB-Fc treatment alone. We also identified several pathways and proteins that were affected by exercise and sActRIIB-Fc together or independently.
Overall design Total RNA obtained from gastrocnemius muscle of mdx mice divided into four groups:
1) control (injected with PBS, n=5), 2) runners (voluntary wheel running for 7 weeks, injected with PBS, n=5), 3) sActRIIB-Fc -treated (n=5), and 4) runners with sActRIIB-Fc-treatment (n=5).
sActRIIB-Fc or PBS was injected intraperitoneally once a week with a 5-mg/kg dose of sActRIIB-Fc. In addition, wildtype mice served as healthy controls (n=4).
Contributor(s) Hulmi JJ, Kainulainen H, Papaioannou KG, Silvennoinen M, Saarela J, Oliveira BM, Nyqvist M, Pasternack A, ’t Hoen PA, Kujala UM, Ritvos O, Autio R
Citation(s) 25304272
Submission date Nov 26, 2013
Last update date Jun 14, 2018
Contact name Juha J Hulmi
Organization name University of Jyväskylä
Department Biology of Physical Activity
Street address Rautpohjankatu 8a Viv
City Jyväskylä
ZIP/Postal code 40014
Country Finland
Platforms (1)
GPL6885 Illumina MouseRef-8 v2.0 expression beadchip
Samples (24)
GSM1275712 Mdx skeletal muscle, PBS+running for 7 weeks rep 1
GSM1275713 Mdx skeletal muscle, PBS+running for 7 weeks rep 2
GSM1275714 Mdx skeletal muscle, PBS+running for 7 weeks rep 3
BioProject PRJNA229986

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SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE52766_RAW.tar 3.1 Mb (http)(custom) TAR
GSE52766_non_normalized.txt.gz 3.9 Mb (ftp)(http) TXT
Raw data are available on Series record
Processed data included within Sample table

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