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| Status |
Public on Dec 03, 2016 |
| Title |
SETX attenuates the antiviral innate immune response and controls viral biogenesis (ChIP-Seq) |
| Organism |
Homo sapiens |
| Experiment type |
Genome binding/occupancy profiling by high throughput sequencing
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| Summary |
The innate immune response is the first line of defense against pathogens, and factors that control this cellular response represent targets for treating both infectious and inflammatory diseases. Here, we reveal a novel role for the human helicase SETX, also previously implicated in amyotrophic lateral sclerosis (ALS4) and ataxia with oculomotor apraxia (AOA2), in controlling the magnitude of the antiviral response. Cells depleted for SETX and AOA2 patient-derived SETX-null cells show increased expression of antiviral mediators in response to infection. Mechanistically, this effect is achieved through SETX-mediated inhibition of RNAPII transcription of antiviral genes, and depends on SETX helicase activity. Our results suggest that SETX helps maintain the delicate balance between controlling viral infection and avoiding the potentially detrimental effects of an excessive antiviral response. More broadly, the observation that SETX can regulate the transcriptional activity of specific genes may have important implications for disorders where SETX function is compromised.
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| Overall design |
A549 cells that were transfected with either control non-targeting or SETX-specific siRNAs were infected with the Influenza A virus (A/PR/8/34(ΔNS1) strain) at a multiplicity of infection (MOI) of 3. 4 hours post infection, cells were collected and used to prepare ChIP libraries. Uninfected cells were used as controls.
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| Contributor(s) |
Miller MS, Rialdi A, Ho JY, Martinez-Gil L, Moshkina NP, Tilove M, Maestre A, Bequerel O, Feagins AR, Basler C, Fernandez-Sesma A, Lavin M, van Bakel H, Marazzi I |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| Submission date |
Dec 03, 2013 |
| Last update date |
May 15, 2019 |
| Contact name |
Ivan Marazzi |
| Organization name |
Icahn School of Medicine at Mount Sinai
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| Department |
Department of Microbiology
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| Street address |
1468 Madison Avenue
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| City |
New York |
| ZIP/Postal code |
10029 |
| Country |
USA |
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| Platforms (1) |
| GPL16791 |
Illumina HiSeq 2500 (Homo sapiens) |
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| Samples (2) |
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| Relations |
| BioProject |
PRJNA230485 |
| SRA |
SRP033443 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE52936_RAW.tar |
949.5 Mb |
(http)(custom) |
TAR (of BEDGRAPH) |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data provided as supplementary file |
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