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| Status |
Public on Nov 04, 2014 |
| Title |
Integrative AUF1 PAR-CLIP analysis uncovers AUF1 roles in translation and genome integrity (Ribosome) |
| Organism |
Homo sapiens |
| Experiment type |
Non-coding RNA profiling by high throughput sequencing
Other
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| Summary |
We report that AUF1 modulates global mRNA stability and translation, in turn promoting the maintenance of DNA integrity.
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| Overall design |
Please see individual series. In short, For AUF1 PAR-CLIP, the four isoforms of AUF1 (p37, p40, p42, and p45) tagged with a Flag epitope were expressed in HEK293 cells. For total RNA-Seq HEK293 cells were transfected with Control siRNA, AUF1 siRNA, Empty Vector, Flag-AUF1 p37, p40, p42, or p45 as well as WI-38 cells were collected at PDL 15 and 55 and also transfected with Control siRNA, AUF1 siRNA, HuR siRNA. For Ribo-Seq HeLa cells were transfected with Control siRNA, AUF1 siRNA, or HuR siRNA.
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| Contributor(s) |
Yoon J, De S, Hafner M, Srikantan S, Abdelmohsen K, Grammatikakis I, Kim J, Martindale JL, Yang X, Kang M, Wood WH, Barnes J, Hooten NN, Evans MK, Becker KG, Tripathi V, Prasanth KV, Tuschl T, Ingolia NT, Gorospe M |
| Citation(s) |
25366541 |
| Submission date |
Dec 04, 2013 |
| Last update date |
Jun 22, 2020 |
| Contact name |
Supriyo De |
| Organization name |
NIA-IRP, NIH
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| Department |
Laboratory of Genetics and Genomics
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| Lab |
Computational Biology & Genomics Core
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| Street address |
251 Bayview Blvd
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| City |
Baltimore |
| State/province |
Maryland |
| ZIP/Postal code |
21224 |
| Country |
USA |
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| Platforms (1) |
| GPL9115 |
Illumina Genome Analyzer II (Homo sapiens) |
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| Samples (3) |
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| This SubSeries is part of SuperSeries: |
| GSE52977 |
Integrative AUF1 PAR-CLIP analysis uncovers AUF1 roles in translation and genome integrity |
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| Relations |
| BioProject |
PRJNA230610 |
| SRA |
SRP033503 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE52976_RAW.tar |
12.2 Mb |
(http)(custom) |
TAR (of TXT) |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data provided as supplementary file |
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