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| Status |
Public on Feb 01, 2014 |
| Title |
Gene expression profiles of wild type and Actinin 4 point mutant knock-in adult podocyte cells at 2, 5 and 44 weeks following TRAP purification. (GUDMAP Series ID: 49) |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
|
| Summary |
The long term goal is to create an encyclopaedia of podocyte gene expression both in homeostasis, development and disease. Podocytes are identified by their expression of an eGFPL10a fusion protein whose expression is driven by a Col1a1 promoter fragment. Podocyte-specific RNA is generated by eGFP-affinity purification of polysomes and then subject to microarray analysis.
Expression profiling of wild type and Actinin 4 point mutant knock-in kidney podocytes by TRAP.
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| Overall design |
Col1a1-eGFPL10a mice were crossed against Actn4+/tm1.1Plk (KI) mice. Wild-type or KI mice were subject to TRAP at the following time points: 2, 5 and 44 weeks.
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| Contributor(s) |
Humphreys BD |
| Citation(s) |
24569379 |
| Submission date |
Dec 10, 2013 |
| Last update date |
Aug 06, 2018 |
| Contact name |
GUDMAP Developers |
| E-mail(s) |
gudmap-db@gudmap.org
|
| Phone |
+44 131 651 8500
|
| Organization name |
IGMM MRC Human Genetics Unit
|
| Lab |
GUDMAP Database Group
|
| Street address |
Crewe Road
|
| City |
Edinburgh |
| ZIP/Postal code |
EH4 2XU |
| Country |
United Kingdom |
| |
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| Platforms (1) |
| GPL11180 |
[HT_MG-430_PM] Affymetrix HT MG-430 PM Array Plate |
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| Samples (25)
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| Relations |
| BioProject |
PRJNA231023 |
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