|
| Status |
Public on Jun 20, 2014 |
| Title |
Analysis of the expression of Ataxin-2-target genes |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
|
| Summary |
We recently identified the RNA targets of Ataxin-2 by using PAR-CLIP. To elucidate the functional role of Ataxin-2 on target-binding, we have knocked down or overexpressed Ataxin-2 in HEK293T cells and extracted RNAs 48 hrs after transfection.
Then, these total RNAs were subjected to whole transcripts microarray analysis to examine how the perturbation of Ataxin-2 expression affects target gene expression.
|
| |
|
| Overall design |
Ataxin-2 was either knocked down using siRNA or overexpressed using the expression construct of Halo-Ataxin-2.Control siRNA was transfected as a control of knockdown experiment.
The expression construct of Halo-tag alone was used as a control of overexpression experiment. Forty-eight hrs after transfection, total RNAs were extracted and subjected to microarray analysis.
|
| |
|
| Contributor(s) |
Yamamoto M, Yokoshi M, Kawahara Y |
| Citation(s) |
24954906 |
| Submission date |
Dec 10, 2013 |
| Last update date |
Jan 09, 2018 |
| Contact name |
Yukio Kawahara |
| E-mail(s) |
ykawahara@rna.med.osaka-u.ac.jp
|
| Phone |
+81-6-6879-3827
|
| Organization name |
Osaka University
|
| Department |
Graduate School of Medicine
|
| Lab |
Laboratory of RNA Function
|
| Street address |
2-2 Yamada-oka
|
| City |
Suita |
| State/province |
Osaka |
| ZIP/Postal code |
565-0871 |
| Country |
Japan |
| |
|
| Platforms (1) |
| GPL17077 |
Agilent-039494 SurePrint G3 Human GE v2 8x60K Microarray 039381 (Probe Name version) |
|
| Samples (12)
|
|
| Relations |
| BioProject |
PRJNA231072 |
Less...
More...