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Series GSE53200 Query DataSets for GSE53200
Status Public on Dec 10, 2013
Title Expression data from OT-I CD8 T cells activated with DC in the presence or absence of CpG-Induced Inflammation
Organism Mus musculus
Experiment type Expression profiling by array
Summary Inflammatory cytokines promote the accumulation of activated CD8 T cells. Here, we transfer 600 OT-I CD8 T cells iv into naïve C57BL/6 hosts. One day later, 500,000 LPS-matured and OVA257 peptide-coated DC were injected iv into OT-I CD8 T cell seeded hosts with (DC+CpG) or without (DC). Other seeded mice were infected with 2x10^4 virulent Listeria monocytogenes (vLM-OVA) iv. OT-I CD8 T cells were harvested from the spleen, flow sort purified, then RNA was extracted using RNeasy (Qiagen) kit. Naive OT-I CD8 T cells (Naive) were purified from the spleens of OT-I transgenic mice. Each group had three independent biological replicates.Transcriptomes were compared using DAVID analysis (with genes scoring FDR<0.01) and GSEA analysis.
 
Overall design 3 biological replicates per group. Groups included Naïve OT-I CD8 T cells, DC+CpG OT-I CD8 T cells, DC OT-I CD8 T cells, and vLM-OVA OT-I CD8 T cells. Most comparisons used Naïve OT-I CD8 T cells as a baseline comparison
 
Contributor(s) Starbeck-Miller GR, Bair T, Hauser G, Harty JT
Citation(s) 24367005
Submission date Dec 10, 2013
Last update date Mar 06, 2018
Contact name John T Harty
E-mail(s) john-harty@uiowa.edu
Phone 319-335-9720
Organization name University of Iowa
Department Microbiology
Street address 3-501 Bowen Science Building, 51 Newton Road
City Iowa City
State/province Iowa
ZIP/Postal code 52242
Country USA
 
Platforms (1)
GPL6096 [MoEx-1_0-st] Affymetrix Mouse Exon 1.0 ST Array [transcript (gene) version]
Samples (12)
GSM1287212 Naïve OT-I CD8 T cells, biological rep1
GSM1287213 Naïve OT-I CD8 T cells, biological rep2
GSM1287214 Naïve OT-I CD8 T cells, biological rep3
Relations
BioProject PRJNA231091

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Supplementary file Size Download File type/resource
GSE53200_RAW.tar 286.7 Mb (http)(custom) TAR (of CEL)
Processed data included within Sample table

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