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GEO help: Mouse over screen elements for information. |
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Status |
Public on Dec 10, 2013 |
Title |
Expression data from OT-I CD8 T cells activated with DC in the presence or absence of CpG-Induced Inflammation |
Organism |
Mus musculus |
Experiment type |
Expression profiling by array
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Summary |
Inflammatory cytokines promote the accumulation of activated CD8 T cells. Here, we transfer 600 OT-I CD8 T cells iv into naïve C57BL/6 hosts. One day later, 500,000 LPS-matured and OVA257 peptide-coated DC were injected iv into OT-I CD8 T cell seeded hosts with (DC+CpG) or without (DC). Other seeded mice were infected with 2x10^4 virulent Listeria monocytogenes (vLM-OVA) iv. OT-I CD8 T cells were harvested from the spleen, flow sort purified, then RNA was extracted using RNeasy (Qiagen) kit. Naive OT-I CD8 T cells (Naive) were purified from the spleens of OT-I transgenic mice. Each group had three independent biological replicates.Transcriptomes were compared using DAVID analysis (with genes scoring FDR<0.01) and GSEA analysis.
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Overall design |
3 biological replicates per group. Groups included Naïve OT-I CD8 T cells, DC+CpG OT-I CD8 T cells, DC OT-I CD8 T cells, and vLM-OVA OT-I CD8 T cells. Most comparisons used Naïve OT-I CD8 T cells as a baseline comparison
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Contributor(s) |
Starbeck-Miller GR, Bair T, Hauser G, Harty JT |
Citation(s) |
24367005 |
Submission date |
Dec 10, 2013 |
Last update date |
Mar 06, 2018 |
Contact name |
John T Harty |
E-mail(s) |
john-harty@uiowa.edu
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Phone |
319-335-9720
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Organization name |
University of Iowa
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Department |
Microbiology
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Street address |
3-501 Bowen Science Building, 51 Newton Road
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City |
Iowa City |
State/province |
Iowa |
ZIP/Postal code |
52242 |
Country |
USA |
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Platforms (1) |
GPL6096 |
[MoEx-1_0-st] Affymetrix Mouse Exon 1.0 ST Array [transcript (gene) version] |
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Samples (12)
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GSM1287212 |
Naïve OT-I CD8 T cells, biological rep1 |
GSM1287213 |
Naïve OT-I CD8 T cells, biological rep2 |
GSM1287214 |
Naïve OT-I CD8 T cells, biological rep3 |
GSM1287215 |
OT-I CD8 T cells from DC-OVA alone immunized mice, biological rep1 |
GSM1287216 |
OT-I CD8 T cells from DC-OVA alone immunized mice, biological rep2 |
GSM1287217 |
OT-I CD8 T cells from DC-OVA alone immunized mice, biological rep3 |
GSM1287218 |
OT-I CD8 T cells from DC-OVA +CpG immunized mice, biological rep1 |
GSM1287219 |
OT-I CD8 T cells from DC-OVA +CpG immunized mice, biological rep2 |
GSM1287220 |
OT-I CD8 T cells from DC-OVA +CpG immunized mice, biological rep3 |
GSM1287221 |
OT-I CD8 T cells from vLM-OVA infected mice, biological rep1 |
GSM1287222 |
OT-I CD8 T cells from vLM-OVA infected mice, biological rep2 |
GSM1287223 |
OT-I CD8 T cells from vLM-OVA infected mice, biological rep3 |
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Relations |
BioProject |
PRJNA231091 |
Supplementary file |
Size |
Download |
File type/resource |
GSE53200_RAW.tar |
286.7 Mb |
(http)(custom) |
TAR (of CEL) |
Processed data included within Sample table |
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