 |
 |
GEO help: Mouse over screen elements for information. |
|
| Status |
Public on Jan 01, 2014 |
| Title |
Nanog Independent Reprogramming to iPSCs with Canonical Factors |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
It has been suggested that the transcription factor Nanog is essential for the establishment of pluripotency during the derivation of embryonic stem (ES) cells and induced pluripotent stem (iPS) cells. However, successful reprogramming to pluripotency with a growing list of divergent transcription factors, at ever increasing efficiencies, suggests that there may be many distinct routes to a pluripotent state. Here, we have investigated whether Nanog is necessary for reprogramming murine fibroblasts under highly efficient conditions using the canonical reprogramming factors Oct4, Sox2, Klf4 and cMyc. In agreement with prior results, the efficiency of reprogramming Nanog-/- fibroblasts was significantly lower than that of control fibroblasts. However, in contrast to previous findings, we were able to reproducibly generate iPS cells from Nanog-/- fibroblasts that effectively contributed to chimeric mice. Thus while Nanog may be an important mediator of reprogramming it is not required for establishing pluripotency in the mouse, even under standard conditions. In order to further evaluate the equivalency of Nanog null iPSC to nanog null ESCs, we have performed RNAseq on two independent nanog null iPSC lines, as well as Nanog Null ESC, WT ESC and iPSCs as well as MEFs. As a negativve control for reprogramming we have analyzed a partially reprogrammed iPSC line.
|
| |
|
| Overall design |
2-4 biological replicates each of 7 conditions (WT MEFs, WT ESC, WT iPSC, WT partially reprogrammed iPSC (piPS), Nanog null ESC, Nanog null iPSC clone G2 and Nanog null iPSC clone G5)
|
| |
|
| Contributor(s) |
Davis-Dusenbery BN, Eggan K |
| Citation(s) |
24527385 |
| Submission date |
Dec 11, 2013 |
| Last update date |
May 15, 2019 |
| Contact name |
Brandi Nicole Davis-Dusenbery |
| Organization name |
Harvard University
|
| Department |
SCRB
|
| Lab |
Kevin Eggan Laboratory
|
| Street address |
7 Divinity Ave
|
| City |
Cambridge |
| State/province |
Massachusetts |
| ZIP/Postal code |
02125 |
| Country |
USA |
| |
|
| Platforms (2) |
| GPL13112 |
Illumina HiSeq 2000 (Mus musculus) |
| GPL17021 |
Illumina HiSeq 2500 (Mus musculus) |
|
| Samples (17)
|
|
| Relations |
| BioProject |
PRJNA231119 |
| SRA |
SRP033700 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE53212_FPKM_matrix.txt.gz |
1.9 Mb |
(ftp)(http) |
TXT |
| GSE53212_RAW.tar |
6.9 Mb |
(http)(custom) |
TAR (of TXT) |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data provided as supplementary file |
| Processed data are available on Series record |
|
|
|
|
 |
Less...
More...