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| Status |
Public on May 02, 2014 |
| Title |
H3K27me3 ChIP-seq experiment in fruitfly testis somatic gonadal cells |
| Organism |
Drosophila melanogaster |
| Experiment type |
Genome binding/occupancy profiling by high throughput sequencing
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| Summary |
In many metazoans, germ cells are separated from somatic lineages early in development and maintain their identity throughout life. Here we show that a Polycomb group (PcG) component, Enhancer of Zeste [E(z)] H3K27me3-specific methyltransferase, maintains germline identity in Drosophila adult testes. We find excessive early-stage somatic gonadal cells in E(z) mutant testes, which originate from both over-proliferative cyst stem cells and germ cells turning on an early-stage somatic cell marker. Using complementary lineage-tracing experiments in E(z) mutant testes, a portion of excessive early-stage somatic gonadal cells are found to derive from early-stage germ cells, including germline stem cells. Interestingly, knocking down E(z) specifically in somatic cells caused this germline-to-soma change, suggesting a non-cell autonomous role of E(z) to antagonize somatic identity in germ cells.
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| Overall design |
Using fly testis specifically expressing E(z) shmiR RNAi in germ cells by nos promoter driven GAL4>UAS system, ChIPseq with H3K27me3 antibody was performed, where H3K27me3 is only detected in somatic cells.
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| Contributor(s) |
Eun SH, Shi Z, Cui K, Zhao K, Chen X |
| Citation(s) |
24675960 |
| Submission date |
Dec 11, 2013 |
| Last update date |
May 15, 2019 |
| Contact name |
Xin Chen |
| E-mail(s) |
xchen32@jhu.edu
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| Organization name |
The Joihns Hopkins University
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| Department |
Biology
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| Lab |
Chen
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| Street address |
3400 N Charles St
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| City |
baltimore |
| State/province |
MD |
| ZIP/Postal code |
21218 |
| Country |
USA |
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| Platforms (1) |
| GPL13304 |
Illumina HiSeq 2000 (Drosophila melanogaster) |
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| Samples (3) |
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| Relations |
| BioProject |
PRJNA231212 |
| SRA |
SRP033728 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE53238_RAW.tar |
2.1 Mb |
(http)(custom) |
TAR (of WIG) |
| GSE53238_chr_read_count_mean.txt.gz |
479 b |
(ftp)(http) |
TXT |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data provided as supplementary file |
| Processed data are available on Series record |
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