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Series GSE53288 Query DataSets for GSE53288
Status Public on Dec 14, 2013
Title Effect of light on gene expression in the zebrafish pineal gland
Organism Danio rerio
Experiment type Expression profiling by array
Summary Microarray data allowed detection of genes that are induced by light in the zebrafish pineal gland
Overall design Adult (0.5-1.5 years old) transgenic zebrafish, Tg(aanat2:EGFP)Y8, which express enhanced green fluorescent protein (EGFP) in the pineal gland under the control of the aanat2 regulatory regions, were used. Fish were raised under 12-hr light:12-hr dark (LD) cycles, in a temperature controlled room. Fish were transferred to constant darkness (DD) at the end of the day prior to the experiment. Fish were exposed to a 1-hr light pulse (light intensity of 12 W/m2) prior to sampling (light treatment) or kept under constant darkness for control (dark treatment). The tissues were collected from light- and dark-treated fish at 6 time points with 4-hr intervals throughout one daily cycle, corresponding to CT2, 6, 10, 14, 18 and 22. Fish were anesthetized in 1.5 mM Tricane (Sigma), sacrificed by decapitation, and pineal glands were removed under a fluorescent dissecting microscope. Pools of 12 pineal glands were prepared at each condition and total RNA was extracted using the RNeasy Lipid Tissue Mini Kit (QIAGEN), according to the manufacturer's instructions.
Contributor(s) Gothilf Y
Citation(s) 24423866
Submission date Dec 13, 2013
Last update date Jan 25, 2018
Contact name Shahar Alon
Organization name Tel Aviv University
Department Department of Neurobiology, The George S. Wise Faculty of Life Sciences
Lab Yoav Gothilf Lab
Street address Tel Aviv University
City Tel Aviv
ZIP/Postal code 69978
Country Israel
Platforms (1)
GPL1319 [Zebrafish] Affymetrix Zebrafish Genome Array
Samples (12)
GSM1288958 Pineal_Dark_CT2
GSM1288959 Pineal_Dark_CT6
GSM1288960 Pineal_Dark_CT10
BioProject PRJNA231567

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SOFT formatted family file(s) SOFTHelp
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Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE53288_RAW.tar 24.9 Mb (http)(custom) TAR (of CEL)
Raw data provided as supplementary file
Processed data included within Sample table

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