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Series GSE53433 Query DataSets for GSE53433
Status Public on May 31, 2015
Title MiRNA profiling in aortas of Apoe-/- mice with an specific knock-out of Dicer in endothelial cells compared to control mice after 12 weeks of a high-fat diet
Organism Mus musculus
Experiment type Expression profiling by RT-PCR
Summary Increased monocyte adhesion to dysfunctional endothelial cells (ECs) orchestrated by chemokines plays an important role in arterial inflammation during atherosclerosis. Endothelial microRNAs (miRNAs) processed by the RNase Dicer1 determine the phenotype of ECs by posttranscriptional regulation of gene expression. However, the impact of endothelial miRNAs on endothelial inflammation and atherosclerosis is currently unclear. To study the effect of Dicer-dependent miRNAs in ECs on atherosclerosis, Apoe-/- mice with an inducible, EC-specific knock-out of Dicer (EC-Dicerflox) and control mice (EC-DicerWT) mice were treated with tamoxifen to induce Cre-recombinase activity and fed with a high fat-diet (HFD) for 12 weeks. The comparison of the miRNA expression profile in the aortas of EC-Dicerflox and EC-DicerWT mice after 12 weeks of a HFD was performed to identify EC-specific miRNAs that may play a role in the EC function during atherogenesis.
Overall design VE-Cadherin-CreERT2 mice were crossed with Dicer1flox/flox/Apoe-/- to obtain VE-Cadherin-CreERT2Dicer1WT/flox/Apoe-/-. VE-Cadherin-CreERT2Dicer1flox/flox/Apoe-/- (EC-Dicerflox) mice and VE-Cadherin-CreERT2Dicer1WT/WT/Apoe-/- (EC-DicerWT) littermates were used for experiments. Cre-recombinase activity was induced by Tamoxifen (TMX) treatment (2 mg/20 g body weight, dissolved in neutral oil) for 5 consecutive days (i.p. injection). One week after the last TMX injection, 6-8 week old females were fed a high-fat diet (HFD) comprising 21% crude fat, 0.15% cholesterol and 19.5% casein for 12 weeks. The aortas were harvested after in situ perfusion with RNAlater and total RNA was isolated using mirVana microRNA Isolation Kit. The Dicer knockout in EC-Dicerflox mice was confirmed by quantification of Dicer1 mRNA expression in the aortas of EC-DicerWT and EC-Dicerflox by qRT-PCR.
Contributor(s) Schober A, Hartmann P, Zhe Z
Citation(s) 26837267
Submission date Dec 18, 2013
Last update date Oct 18, 2019
Contact name Andreas Schober
Organization name Ludwig-Maximilians-University
Department Institute for Cardiovascular Prevention
Street address Pettenkoferstr. 9
City Munich
State/province Bavaria
ZIP/Postal code 80336
Country Germany
Platforms (1)
GPL18082 Applied Biosystems Taqman Array Rodent MicroRNA Cards v3.0
Samples (6)
GSM1293407 aorta of EC-Dicerflox mice Replicate 1 (12wks)
GSM1293408 aorta of EC-Dicerflox mice Replicate 2 (12wks)
GSM1293409 aorta of EC-Dicerflox mice Replicate 3 (12wks)
BioProject PRJNA231985

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Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE53433_ct_raw_data.txt.gz 12.0 Kb (ftp)(http) TXT
Raw data are available on Series record
Processed data included within Sample table

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