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Series GSE53497 Query DataSets for GSE53497
Status Public on Dec 17, 2016
Title First round of DNA replication is essential for transcriptional reprogramming of somatic nuclei in mouse oocytes
Organism Mus musculus
Experiment type Expression profiling by array
Summary Many of the structural and mechanistic requirements of oocyte-mediated nuclear reprogramming remain elusive. Previous accounts that transcriptional reprogramming of somatic nuclei in mouse zygotes may be complete in 24-36 hours, far more rapidly than in other reprogramming systems, raise the question of whether the mere exposure to the activated mouse ooplasm is sufficient to enact reprogramming in a nucleus. We therefore prevented DNA replication and cytokinesis, which ensue after nuclear transfer, in order to assess their requirement for transcriptional reprogramming of the key pluripotency genes Oct4 (Pou5f1) and Nanog in cloned mouse embryos. Using transcriptome and allele-specific analysis, we observed that hundreds of mRNAs, but not Oct4 and Nanog, became elevated in nucleus-transplanted oocytes without DNA replication. Progression through the first round of DNA replication was essential but not sufficient for transcriptional reprogramming of Oct4 and Nanog, whereas cytokinesis and thereby cell-cell interactions were dispensable for transcriptional reprogramming. Responses similar to clones also were observed in embryos produced by fertilization in vitro. Our results link the occurrence of reprogramming to a previously unappreciated requirement of oocyte-mediated nuclear reprogramming, namely DNA replication. Nuclear transfer alone affords no immediate transition from a somatic to a pluripotent gene expression pattern unless DNA replication is also in place. This study is therefore a resource to appreciate that the quest for always faster reprogramming methods may collide with a limit that is dictated by the cell cycle.
 
Overall design 13 samples were analyzed.
Aph-IVF: Aphidicolin not treated, in vitro fertilization, 1 biological rep
Aph-IVF96: Aphidicolin not treated, in vitro fertilization (96h), 1 biological rep
Aph-IVFBla: Aphidicolin not treated, in vitro fertilization blastocyst, 1 biological rep
Aph+IVF: Aphidicolin treated, in vitro fertilization, 1 biological rep
Aph+IVF96: Aphidicolin treated, in vitro fertilization (96h), 1 biological rep
Aph+IVFBla: Aphidicolin treated, in vitro fertilization blastocyst, 1 biological rep
Aph-SCNT: Aphidicolin not treated, somatic cell nuclear transfer, 1 biological rep
Aph-SCNT96: Aphidicolin not treated, somatic cell nuclear transfer (96h), 1 biological rep
Aph-SCNTBla: Aphidicolin not treated, somatic cell nuclear transfer blastocyst, 1 biological rep
Aph+SCNTBla: Aphidicolin treated, somatic cell nuclear transfer blastocyst, 1 biological rep
B6C3F1-Ooc: Aphidicolin not treated, B6C3F1 oocytes, 1 biological rep
B6C3F1-Cum: Aphidicolin not treated, B6C3F1 cumulus cells, 2 biological rep
 
Contributor(s) Wang B, Pfeiffer MJ, Schwarzer C, Arauzo-Bravo MJ, Boiani M
Citation(s) 24836291
Submission date Dec 19, 2013
Last update date Sep 05, 2019
Contact name Marcos J Ara├║zo-Bravo
E-mail(s) mararabra@yahoo.co.uk
Organization name Max Planck Institute for Molecular Biomedicine
Department Cell and Developmental Biology
Lab Computational Biology and Bioinformatics
Street address Rontgenstr. 20
City Muenster
ZIP/Postal code 48149
Country Germany
 
Platforms (1)
GPL6885 Illumina MouseRef-8 v2.0 expression beadchip
Samples (13)
GSM1294947 Aph-IVF rep1
GSM1294948 Aph-IVF96 rep1
GSM1294949 Aph-IVFBla rep1
Relations
BioProject PRJNA232136

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE53497_RAW.tar 3.1 Mb (http)(custom) TAR
GSE53497_non-normalized.txt.gz 2.1 Mb (ftp)(http) TXT
Raw data are available on Series record
Processed data included within Sample table

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