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GEO help: Mouse over screen elements for information. |
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| Status |
Public on Apr 06, 2015 |
| Title |
Human PGC commitment shares key gene dynamics to mouse, but owns a unique PRDM14 expression pattern |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
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| Summary |
The molecular mechanisms of human primordial germ cell (PGC) specification are poorly understood due
to the inaccessibility of cell materials and the lack of an alternative in vitro model that enables
tracking of the earliest stages of germ cell development.
Here, we introduce a defined and efficient differentiation system for the induction of pre-migratory PGC-like cells
from human embryonic stem cells (ESCs)
and induced pluripotent stem cells (iPSCs).
By step-wise differentiation, we generated an OCT4+/ T+/BLIMP1+ cell population that transitioned into
STELLA expressing PGC-like cells
that exhibited a similar key gene expression as mouse PGCs as well as global epigenetic reprogramming.
Even though, these PGC-like cells expressed PRDM14 at very low levels,
they underwent activation of pluripotency/PGC genes,
suppression of neural induction and suppression of de novo DNA methylation,
events that are regulated by Prdm14 during mouse PGC specification.
This study demonstrates that human PGC commitment shares many key features with mouse PGC specification,
but harbors unique and so far unknown mechanisms that,
point to a novel human transcriptional regulation.
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| Overall design |
7 samples were analyzed.
ESC: Human Embryonic Stem Cells, 1 biological rep
iPSC: Human induced Pluripotent Stem Cells, 1 biological rep
d2: Human induced Pluripotent Stem Cells, 2 days differentiation treatment , 2 biological rep
d4PGCLC: Human induced Pluripotent Stem Cells, 4 days differentiation treatment towards Primordial Germ Cells Like Cells, 1 biological rep
d6PGCLC: Human induced Pluripotent Stem Cells, 6 days differentiation treatment towards Primordial Germ Cells Like Cells, 2 biological rep
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| Contributor(s) |
Sugawa F, Arauzo-Bravo MJ, Kim K, Stehling M, Hubner K, Scholer HR |
| Citation(s) |
25750208 |
| Submission date |
Dec 19, 2013 |
| Last update date |
Jan 15, 2022 |
| Contact name |
Marcos J. Araúzo-Bravo |
| E-mail(s) |
mararabra@yahoo.co.uk
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| Phone |
+34 943 00 6108
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| Organization name |
Max Planck Institute for Molecular Biomedicine
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| Department |
Cell and Developmental Biology
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| Lab |
Computational Biology and Bionformatics
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| Street address |
Rogentstrasse
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| City |
Muenster |
| ZIP/Postal code |
48149 |
| Country |
Germany |
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| Platforms (1) |
| GPL10558 |
Illumina HumanHT-12 V4.0 expression beadchip |
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| Samples (7)
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| Relations |
| BioProject |
PRJNA232135 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE53498_RAW.tar |
26.2 Mb |
(http)(custom) |
TAR |
| GSE53498_non-normalized.txt.gz |
2.1 Mb |
(ftp)(http) |
TXT |
| Processed data included within Sample table |
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