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Status |
Public on Aug 01, 2014 |
Title |
Effect of in utero undernutrition on skeletal muscle gene expression |
Organism |
Mus musculus |
Experiment type |
Expression profiling by array
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Summary |
In utero undernutrition is associated with obesity and insulin resistance, although its effect on skeletal muscle remains poorly defined. We report that, in mice, adult offspring from undernourished dams have decreased energy expenditure, decreased skeletal muscle mitochondrial content, and altered energetics in isolated mitochondria and permeabilized muscle fibers. Strikingly, when these mice are put on a 40% calorie restricted diet they lose half as much weight as calorie restricted controls. Our results reveal for the first time that in utero undernutrition alters metabolic physiology having a profound effect on skeletal muscle energetics and response to calorie restriction in adulthood.
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Overall design |
We have used a mouse model of low birth weight generated through 50% food restriction of mouse dams during the third week of gestation. We have studied in utero food restricted offspring and control offspring that were not food restricted in utero in both the ad libitum and calorie restricted states. Gene expression profiling was performed on tibialis anterior muscle from 8 mice per group, pooled in pairs.
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Contributor(s) |
Beauchamp BL, Ghosh S, Chu A, Blais A, Harper M |
Citation(s) |
25091727 |
Submission date |
Dec 19, 2013 |
Last update date |
May 10, 2023 |
Contact name |
Alexandre Blais |
E-mail(s) |
alexandre.blais@uottawa.ca
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Organization name |
University of Ottawa
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Street address |
451 Smyth Road
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City |
Ottawa |
State/province |
ON |
ZIP/Postal code |
K1H 8M5 |
Country |
Canada |
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Platforms (1) |
GPL7202 |
Agilent-014868 Whole Mouse Genome Microarray 4x44K G4122F (Probe Name version) |
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Samples (16)
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GSM1295431 |
tibialis anterior-MotherDiet_Control-OffspringDiet_Ad Lib-Rep1 |
GSM1295432 |
tibialis anterior-MotherDiet_Control-OffspringDiet_Ad Lib-Rep2 |
GSM1295433 |
tibialis anterior-MotherDiet_Control-OffspringDiet_Ad Lib-Rep3 |
GSM1295434 |
tibialis anterior-MotherDiet_Control-OffspringDiet_Ad Lib-Rep4 |
GSM1295435 |
tibialis anterior-MotherDiet_Control-OffspringDiet_Colorie Restricted-Rep1 |
GSM1295436 |
tibialis anterior-MotherDiet_Control-OffspringDiet_Colorie Restricted-Rep2 |
GSM1295437 |
tibialis anterior-MotherDiet_Control-OffspringDiet_Colorie Restricted-Rep3 |
GSM1295438 |
tibialis anterior-MotherDiet_Control-OffspringDiet_Colorie Restricted-Rep4 |
GSM1295439 |
tibialis anterior-MotherDiet_Undernourished-OffspringDiet_Ad Lib-Rep1 |
GSM1295440 |
tibialis anterior-MotherDiet_Undernourished-OffspringDiet_Ad Lib-Rep2 |
GSM1295441 |
tibialis anterior-MotherDiet_Undernourished-OffspringDiet_Ad Lib-Rep3 |
GSM1295442 |
tibialis anterior-MotherDiet_Undernourished-OffspringDiet_Ad Lib-Rep4 |
GSM1295443 |
tibialis anterior-MotherDiet_Undernourished-OffspringDiet_Colorie Restricted-Rep1 |
GSM1295444 |
tibialis anterior-MotherDiet_Undernourished-OffspringDiet_Colorie Restricted-Rep2 |
GSM1295445 |
tibialis anterior-MotherDiet_Undernourished-OffspringDiet_Colorie Restricted-Rep3 |
GSM1295446 |
tibialis anterior-MotherDiet_Undernourished-OffspringDiet_Colorie Restricted-Rep4 |
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Relations |
BioProject |
PRJNA232265 |