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| Status |
Public on Nov 15, 2014 |
| Title |
Expression analysis of blastocysts in Kdm4b-PEs |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
|
| Summary |
To identify the genes that are controled by H3K9me3 modification in PEs, we conducted transcriptome analysis using Egfp-, Kdm4b-PEs, and IVF embryos at blastocyst stages.
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| |
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| Overall design |
Kdm4b or Egfp mRNA injected oocytes were parthenogenetically activated. IVF embryos were generated according to standard protcol. All embryos were cultured in KSOM medium for 120 hours after activation or sperm insemination. Five blastocysts per one biological replicate were pooled and analyzed.
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| |
|
| Contributor(s) |
Fukuda A |
| Citation(s) |
25394724 |
| Submission date |
Dec 26, 2013 |
| Last update date |
Feb 02, 2018 |
| Contact name |
Atsushi Fukuda |
| E-mail(s) |
fukuda-at@ncchd.go.jp
|
| Organization name |
National Center for Child Health and Development
|
| Department |
Reproductive biology
|
| Street address |
2-10-1 Okura
|
| City |
Setagaya-ku |
| State/province |
Tokyo |
| ZIP/Postal code |
157-8535 |
| Country |
Japan |
| |
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| Platforms (1) |
| GPL10787 |
Agilent-028005 SurePrint G3 Mouse GE 8x60K Microarray (Probe Name version) |
|
| Samples (8)
|
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| Relations |
| BioProject |
PRJNA233656 |
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