|
| Status |
Public on Dec 30, 2013 |
| Title |
Mouse Endothelial Progenitor Cell (EPC) Lines - Illumnia Array |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
|
| Summary |
Mouse EPCs were harvested from lung tissue and cultured in hypoxic chambers (2% FiO2) for 48 hrs and then transferred to regular incubators. Cell lines were then stained for the uptake of the acetylated form of low density lipoprotein. These cells were then sorted by flow cytometry and labeled as EPC_acLDL cells. Finally, high passage EPC_acLDL cells (p16-20) were collected and labeled as EPC_late cells. The three cell lines were then grown up, harvested, and RNA isolated and submitted in duplicate for Illumina array analysis of gene expression.
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| |
|
| Overall design |
3 samples, in duplicate: EPC, EPC_acLDL, and EPC_Late
|
| |
|
| Contributor(s) |
Russell JS |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| Submission date |
Dec 27, 2013 |
| Last update date |
Jun 14, 2018 |
| Contact name |
Jeff Russell |
| E-mail(s) |
russelljs73@gmail.com
|
| Phone |
813-745-8118
|
| Organization name |
Moffitt Cancer Center
|
| Department |
Head and Neck/Endocrine
|
| Street address |
12902 Magnolia Drive
|
| City |
Tampa |
| State/province |
FL |
| ZIP/Postal code |
33612 |
| Country |
USA |
| |
|
| Platforms (1) |
| GPL6885 |
Illumina MouseRef-8 v2.0 expression beadchip |
|
| Samples (6)
|
|
| Relations |
| BioProject |
PRJNA232632 |
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