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Series GSE53681 Query DataSets for GSE53681
Status Public on Dec 30, 2013
Title Mouse Endothelial Progenitor Cell (EPC) Lines - Illumnia Array
Organism Mus musculus
Experiment type Expression profiling by array
Summary Mouse EPCs were harvested from lung tissue and cultured in hypoxic chambers (2% FiO2) for 48 hrs and then transferred to regular incubators. Cell lines were then stained for the uptake of the acetylated form of low density lipoprotein. These cells were then sorted by flow cytometry and labeled as EPC_acLDL cells. Finally, high passage EPC_acLDL cells (p16-20) were collected and labeled as EPC_late cells. The three cell lines were then grown up, harvested, and RNA isolated and submitted in duplicate for Illumina array analysis of gene expression.
 
Overall design 3 samples, in duplicate: EPC, EPC_acLDL, and EPC_Late
 
Contributor(s) Russell JS
Citation missing Has this study been published? Please login to update or notify GEO.
Submission date Dec 27, 2013
Last update date Jun 14, 2018
Contact name Jeff Russell
E-mail(s) russelljs73@gmail.com
Phone 813-745-8118
Organization name Moffitt Cancer Center
Department Head and Neck/Endocrine
Street address 12902 Magnolia Drive
City Tampa
State/province FL
ZIP/Postal code 33612
Country USA
 
Platforms (1)
GPL6885 Illumina MouseRef-8 v2.0 expression beadchip
Samples (6)
GSM1298793 EPC rep1
GSM1298794 EPC_acLDL rep1
GSM1298795 EPC_Late rep1
Relations
BioProject PRJNA232632

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE53681_RAW.tar 3.1 Mb (http)(custom) TAR
GSE53681_non_normalized.txt.gz 1.3 Mb (ftp)(http) TXT
Raw data are available on Series record
Processed data included within Sample table

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